The AP-1 transcription factor Batf controls TH17 differentiation (original) (raw)

Nature volume 460, pages 405–409 (2009)Cite this article

Abstract

Activator protein 1 (AP-1, also known as JUN) transcription factors are dimers of JUN, FOS, MAF and activating transcription factor (ATF) family proteins characterized by basic region and leucine zipper domains1. Many AP-1 proteins contain defined transcriptional activation domains, but BATF and the closely related BATF3 (refs 2, 3) contain only a basic region and leucine zipper, and are considered to be inhibitors of AP-1 activity3,4,5,6,7,8. Here we show that Batf is required for the differentiation of IL17-producing T helper (TH17) cells9. TH17 cells comprise a CD4+ T-cell subset that coordinates inflammatory responses in host defence but is pathogenic in autoimmunity10,11,12,13. _Batf_-/- mice have normal TH1 and TH2 differentiation, but show a defect in TH17 differentiation, and are resistant to experimental autoimmune encephalomyelitis. _Batf_-/- T cells fail to induce known factors required for TH17 differentiation, such as RORγt11 (encoded by Rorc) and the cytokine IL21 (refs 14–17). Neither the addition of IL21 nor the overexpression of RORγt fully restores IL17 production in _Batf_-/- T cells. The Il17 promoter is BATF-responsive, and after TH17 differentiation, BATF binds conserved intergenic elements in the Il17a–Il17f locus and to the Il17, Il21 and Il22 (ref. 18) promoters. These results demonstrate that the AP-1 protein BATF has a critical role in TH17 differentiation.

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ArrayExpress

Data deposits

Microarray data are available at Array Express (http://www.ebi.ac.uk/arrayexpress/) under the accession numbers E-MEXP-1518, E-MEXP-2152 and E-MEXP-2153.

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Acknowledgements

We thank R. Lallone for anti-BATF antibody preparation, and B. Sleckman for Cre-expressing adenovirus. This work was supported by the Howard Hughes Medical Institute (K.M.M.), and grants from the National Institutes of Health HG00249 and training grant GM07200 (G.D.S.), AI035783 (C.T.W.), AR049293 (R.D.H.), and from Daiichi-Sankyo Co. Ltd (C.T.W.).

Author Contributions B.U.S. generated _Batf_-/- mice, designed and analysed the experiments, interpreted results and wrote the manuscript. K.H. constructed the targeting vector and probes, transgenic vector, and recombinant BATF. W.I. helped with retroviral expression experiments. W.-L.L. helped with reverse-strand reporter analysis. W.A.-E.S. helped with mouse generation. B.S. helped with EMSA analysis. G.S. and G.D.S. performed bioinformatics analysis for the BATF binding elements. J.S. and J.H.R. helped with EAE experiments. R.M., R.D.H. and C.T.W. performed ChIP experiments. T.L.M. and S.C. performed confocal microscopy for BATF. K.M.M. directed the study and wrote the manuscript.

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Author notes

  1. Barbara U. Schraml and Kai Hildner: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Pathology and Immunology,,
    Barbara U. Schraml, Kai Hildner, Wataru Ise, Wan-Ling Lee, Whitney A.-E. Smith, Ben Solomon, Saso Cemerski, Theresa L. Murphy & Kenneth M. Murphy
  2. Howard Hughes Medical Institute,,
    Kai Hildner, Wataru Ise & Kenneth M. Murphy
  3. Department of Genetics,,
    Gurmukh Sahota & Gary D. Stormo
  4. Department of Molecular Biology and Pharmacology, Washington University School of Medicine, 660 South Euclid Avenue, Saint Louis, Missouri 63110, USA,
    Julia Sim & John H. Russell
  5. Department of Pathology, University of Alabama at Birmingham, University Station, Birmingham, Alabama 35294, USA,
    Ryuta Mukasa, Robin D. Hatton & Casey T. Weaver

Authors

  1. Barbara U. Schraml
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  2. Kai Hildner
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  3. Wataru Ise
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  4. Wan-Ling Lee
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  5. Whitney A.-E. Smith
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  6. Ben Solomon
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  7. Gurmukh Sahota
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  8. Julia Sim
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  9. Ryuta Mukasa
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  10. Saso Cemerski
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  11. Robin D. Hatton
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  12. Gary D. Stormo
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  13. Casey T. Weaver
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  14. John H. Russell
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  15. Theresa L. Murphy
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  16. Kenneth M. Murphy
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Corresponding author

Correspondence toKenneth M. Murphy.

Supplementary information

Supplementary Information

This file contains Supplementary Figures 1-14 with Legends, Supplementary Tables 1, 5-6, Supplementary Methods and Supplementary References. (PDF 1930 kb)

Supplementary Table 2

This file contains microarray data in Excel format accompanying Figure 3c. (XLS 27 kb)

Supplementary Table 3

This file contains microarray data in Excel format accompanying Supplementary Figure 9a. (XLS 1 kb)

Supplementary Table 4

This file contains Gene ChIP data in Excel format accompanying Supplementary Figure 9b. (XLS 1 kb)

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Schraml, B., Hildner, K., Ise, W. et al. The AP-1 transcription factor Batf controls TH17 differentiation.Nature 460, 405–409 (2009). https://doi.org/10.1038/nature08114

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Editorial Summary

New role for AP-1/JUN

AP-1 (activator protein-1) transcription factors, also known as JUN factors, are broadly acting factors regulating many inducible genetic responses. Schraml et al. report a surprising mechanism that expands the biological repertoire of the AP-1 family. They find that the AP-1 transcription factor Batf plays a critical role in the differentiation of IL17-producing T-helper cells.