Construction and Characterization of a Set of E. coli Strains Deficient in All Known Loci Affecting the Proteolytic Stability of Secreted Recombinant Proteins (original) (raw)

References

  1. Georgiou, G. 1994. Expression of proteins in bacteria, p. xxx?. In: Principles and Practice of Protein Engineering. Cleland, J. L. and Craik, C. S. (Eds.). Plenum Press, New York.
    Google Scholar
  2. Stader, J.A. and Silhavy, T.J. 1990. Engineering Escherichia coli to secrete heterologous gene products. Meth. Enzymol. 185: 166–187.
    Article CAS Google Scholar
  3. Baneyx, F. and Georgiou, G. 1992. Expression of proteolytically sensitive polypeptides in Escherichia coli, p. 69–108. In: Stability of Protein Pharmaceuticals, Vol. 3: Chemical and Physical Pathways of Protein Degradation. Ahern, T. J. and Manning, M. C. (Eds.). Plenum Press, New York.
    Google Scholar
  4. Enfors, S.-O. 1992. Control of in vivo proteolysis in the production of recombinant proteins. Trends Biotechnol. 10: 310–315.
    Article CAS PubMed Google Scholar
  5. Lazdunski, A.M. 1989. Peptidases and proteases of Escherichia coli and Salmonella typhimurium. FEMS Microbiol. Rev. 63: 265–276.
    CAS Google Scholar
  6. Maurizi, M.R. 1992. Proteases and protein degradation in Escherichia coli. Experientia 48: 178–201.
    Article CAS PubMed Google Scholar
  7. Strauch, K.L. and Beckwith, J. 1988. An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins. Proc. Natl. Acad. Sci. USA 85: 1576–1580.
    Article CAS PubMed PubMed Central Google Scholar
  8. Lipinska, B., Fayet, O., Baird, L. and Georgopoulos, C. 1989. Identification, characterization, and mapping of the Escherichia coli htrA gene, whose product is essential for bacterial growth only at elevated temperatures. J. Bacteriol. 171: 1574–1584.
    Article CAS PubMed PubMed Central Google Scholar
  9. Lipinska, B., Sharma, S. and Georgopoulos, C. 1988. Sequence analysis and regulation of the htrA gene of Escherichia coli: a σ32-independent mechanism of heat-inducible transcription. Nucleic Acids Res. 16: 10053–10067.
    Article CAS PubMed PubMed Central Google Scholar
  10. Strauch, K.L., Johnson, K. and Beckwith, J. 1989. Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature. J. Bacteriol. 171: 2689–2696.
    Article CAS PubMed PubMed Central Google Scholar
  11. Hara, H., Yamamoto, Y., Higashitani, A., Suzuki, H. and Nishimura, Y. 1991. Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3. J. Bacteriol. 173: 4799–4813.
    Article CAS PubMed PubMed Central Google Scholar
  12. Silber, K.R., Keiler, K.C. and Sauer, R.T. 1992. Tsp: a tail-specific protease that selectively degrades proteins with nonpolar C-termini. Proc. Natl. Acad. Sci. USA 89: 295–299.
    Article CAS PubMed PubMed Central Google Scholar
  13. Baneyx, F. and Georgiou, G. 1991. Construction and characterization of Escherichia coli strains deficient in multiple secreted proteases: protease III degrades high-molecular-weight substrates in vivo. J. Bacteriol. 173: 2696–2703.
    Article CAS PubMed PubMed Central Google Scholar
  14. Gross, C.A., Straus, D.B., Erickson, J.W. and Yura, T. 1990. The function and regulation of heat shock proteins in Escherichia coli, p. 167–189. In: Stress Proteins in Biology and Medicine. Morimoto, R., Tissieres, A. and Georgopoulos, C. (Eds.). Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
    Google Scholar
  15. Goff, S.A. and Goldberg, A.L. 1985. Production of abnormal proteins in E. coli stimulates transcription of Ion and other heat shock genes. Cell 41: 587–595.
    Article CAS PubMed Google Scholar
  16. Goff, S.A., Casson, L.P. and Goldberg, A.L. 1984. Heat shock regulatory gene htpR influences rates of protein degradation and expression of the Ion gene in Escherichia coli. Proc. Natl. Acad. Sci. USA 81: 6647–6651.
    Article CAS PubMed PubMed Central Google Scholar
  17. Neidhardt, F.C. and VanBogelen, R.A. 1987. Heat shock response, p. 1462–1471. In: Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, Vol. 1. Neidhardt, F. C., Ingraham, J. L., Low, K. B., Magasanik, B., Schaechter, M. and Umbarger, H. E. (Eds.). American Society for Microbiology, Washington, D. C.
    Google Scholar
  18. Helmann, J.D. and Chamberlin, M.J. 1988. Structure and function of bacterial sigma factors. Ann. Rev. Biochem. 57: 839–872.
    Article CAS PubMed Google Scholar
  19. Baker, T.A., Grossman, A.D. and Gross, C.A. 1984. A gene regulating the heat shock response in Escherichia coli also affects proteolysis. Proc. Natl. Acad. Sci. USA 81: 6779–6783.
    Article CAS PubMed PubMed Central Google Scholar
  20. Straus, D.B., Walter, W.A. and Gross, C.A. 1988. Escherichia coli heat shock gene mutants are defective in proteolysis. Genes Dev. 2: 1851–1858.
    Article CAS PubMed Google Scholar
  21. Zhou, Y.-N., Kusukawa, N., Erickson, J.W., Gross, C.A. and Yura, T. 1988. Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor σ32. J Bacteriol. 170: 3640–3649.
    Article CAS PubMed PubMed Central Google Scholar
  22. Baneyx, F. and Georgiou, G. 1989. Expression, purification and enzymatic characterization of a protein A-β-lactamase hybrid protein. Enzyme Microb. Technol. 11: 559–567.
    Article CAS Google Scholar
  23. Cooper, S. and Ruettinger, T. 1975. A temperature sensitive nonsense mutation affecting the synthesis of a major protein of Escherichia coli K12. Mol. Gen. Genet. 139: 167–176.
    Article CAS PubMed Google Scholar
  24. Meerman, H.J. 1994. Ph. D. Thesis, The University of Texas at Austin, Austin, TX.
  25. Kusukawa, N., Yura, T., Ueguchi, C., Akiyama, K. and Ito, K. 1989. Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli. EMBO J. 8: 3517–3521.
    Article CAS PubMed PubMed Central Google Scholar
  26. Wild, J., Altman, E., Yura, T. and Gross, C.A. 1992. DnaK and GnaJ heat shock proteins participate in protein export in Escherichia coli. Genes Dev. 6: 1165–1172.
    Article CAS PubMed Google Scholar
  27. Meerman, H.J. and Georgiou, G. 1994. The expression of secreted fusion proteins in Escherichia coli rpoH165 mutants results in increased DegP (HtrA)-mcdiated protein degradation. Submitted.
  28. Yura, T., Tobe, T., Ito, K. and Osawa, T. 1984. Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensible at low temperature. Proc. Natl. Acad. Sci. USA 81: 6803–6808.
    Article CAS PubMed PubMed Central Google Scholar
  29. Tilly, K. 1991. Independence of bacteriophage N15 lytic and linear plasmid replication from the heat shock proteins DnaJ, DnaK, and GrpE. J. Bacteriol. 173: 6639–6642.
    Article CAS PubMed PubMed Central Google Scholar
  30. Maloy, S.R. and Nunn, W.D. 1981. Selection for loss of tetracycline resistance by Escherichia coli. J. Bacteriol. 145: 1110–1112.
    CAS PubMed PubMed Central Google Scholar
  31. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.D., Smith, J.A. and Struhl, K. 1987. Current Protocols in Molecular Biology. John Wiley & Sons, New York.
    Google Scholar

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