Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex (original) (raw)

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Acknowledgements

We thank N. Pace, L. Gold and F. Accurso for support and encouragement, J.I. Gordon and R. Bushman for helpful discussions, and R. Ley, C. Lozupone and D. McDonald for feedback on the manuscript. This work was supported in part by the US National Institutes of Health–University of Colorado at Boulder Molecular Biophysics Training Program (T32GM065103), and grants from the Cystic Fibrosis Foundation and National Institutes of Health (U01 HL081335-01, P01DK078669).

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Authors and Affiliations

  1. Department of Computer Science, UCB 430, University of Colorado, Boulder, 80309, Colorado, USA
    Micah Hamady
  2. Department of Molecular, Cellular and Developmental Biology, UCB 347, University of Colorado, Boulder, 80309, Colorado, USA
    Jeffrey J Walker & Nicholas J Gold
  3. Department of Pediatrics, University of Colorado Denver and Health Sciences Center, Children's Hospital, 13123 E. 16th Avenue Box B395, Aurora, 80045, Colorado, USA
    J Kirk Harris
  4. Department of Chemistry and Biochemistry, UCB 215, University of Colorado, Boulder, 80309, Colorado, USA
    Rob Knight

Authors

  1. Micah Hamady
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  2. Jeffrey J Walker
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  3. J Kirk Harris
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  4. Nicholas J Gold
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  5. Rob Knight
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Contributions

M.H. and R.K. designed and implemented the analyses, and wrote the manuscript. J.J.W., J.K.H. and N.J.G. generated the 454 dataset.

Corresponding author

Correspondence toRob Knight.

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Hamady, M., Walker, J., Harris, J. et al. Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.Nat Methods 5, 235–237 (2008). https://doi.org/10.1038/nmeth.1184

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