Transposon-tagged mutagenesis in the rat (original) (raw)
- Brief Communication
- Published: 14 January 2007
- Satoshi Ishishita1,2,
- Keiko Tosaka1,
- Ri-ichi Takahashi3,
- Masatsugu Ueda3,
- Vincent W Keng4,
- Kyoji Horie5 &
- …
- Junji Takeda4,5
Nature Methods volume 4, pages 131–133 (2007)Cite this article
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Abstract
Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.
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Acknowledgements
We thank P. B. Hackett (University of Minnesota) for providing SB cassettes.
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Authors and Affiliations
- Division of Genome Dynamics, Laboratory of Mammalian Genetics, Creative Research Initiative “Sousei”, Hokkaido University, North 10 West 8, Kita-ku, Sapporo, 060-0810, Japan
Kazuhiro Kitada, Satoshi Ishishita & Keiko Tosaka - Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo, 060-0810, Japan
Satoshi Ishishita - The YS Institute, Inc., 1198-4, Iwazo, Utsunomiya, Tochigi, 321-0973, Japan
Ri-ichi Takahashi & Masatsugu Ueda - Center for Advanced Science and Innovation, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan
Vincent W Keng & Junji Takeda - Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan
Kyoji Horie & Junji Takeda
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- Kazuhiro Kitada
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Correspondence toKazuhiro Kitada.
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Kitada, K., Ishishita, S., Tosaka, K. et al. Transposon-tagged mutagenesis in the rat.Nat Methods 4, 131–133 (2007). https://doi.org/10.1038/nmeth1002
- Received: 04 October 2006
- Accepted: 01 December 2006
- Published: 14 January 2007
- Issue Date: 01 February 2007
- DOI: https://doi.org/10.1038/nmeth1002