Transposon-tagged mutagenesis in the rat (original) (raw)

Nature Methods volume 4, pages 131–133 (2007)Cite this article

Abstract

Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.

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Acknowledgements

We thank P. B. Hackett (University of Minnesota) for providing SB cassettes.

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Authors and Affiliations

  1. Division of Genome Dynamics, Laboratory of Mammalian Genetics, Creative Research Initiative “Sousei”, Hokkaido University, North 10 West 8, Kita-ku, Sapporo, 060-0810, Japan
    Kazuhiro Kitada, Satoshi Ishishita & Keiko Tosaka
  2. Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo, 060-0810, Japan
    Satoshi Ishishita
  3. The YS Institute, Inc., 1198-4, Iwazo, Utsunomiya, Tochigi, 321-0973, Japan
    Ri-ichi Takahashi & Masatsugu Ueda
  4. Center for Advanced Science and Innovation, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan
    Vincent W Keng & Junji Takeda
  5. Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan
    Kyoji Horie & Junji Takeda

Authors

  1. Kazuhiro Kitada
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  2. Satoshi Ishishita
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  3. Keiko Tosaka
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  4. Ri-ichi Takahashi
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  5. Masatsugu Ueda
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  6. Vincent W Keng
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  7. Kyoji Horie
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  8. Junji Takeda
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Corresponding author

Correspondence toKazuhiro Kitada.

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The authors declare no competing financial interests.

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Kitada, K., Ishishita, S., Tosaka, K. et al. Transposon-tagged mutagenesis in the rat.Nat Methods 4, 131–133 (2007). https://doi.org/10.1038/nmeth1002

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