Increasing Adult Hippocampal Neurogenesis is Sufficient to Reduce Anxiety and Depression-Like Behaviors (original) (raw)

INTRODUCTION

Adult hippocampal neurogenesis is a process through which additional granule cells are added to the dentate gyrus throughout life. These cells are produced from progenitors located in the subgranular zone of the dentate gyrus, and their rates of proliferation, maturation, and survival are impacted by environmental conditions such as age, stress, exercise, and antidepressants (Dranovsky et al, 2011; Gould et al, 1997; Malberg et al, 2000; van Praag et al, 2005).

Many antidepressants are known to act through monoamine systems, however the downstream mechanisms through which they affect mood are still not entirely understood. While many antidepressants change monoamine levels within hours, effective changes in mood are not seen for 3–4 weeks. This disparity, along with the findings that antidepressants increase the number of adult-born neurons (Boldrini et al, 2009; Malberg et al, 2000), which take about 4 weeks to form synaptic connections (Toni et al, 2007) and contribute to behavior in rodents (Denny et al, 2012; Kee et al, 2007), led to the hypothesis that antidepressants might affect mood by increasing adult hippocampal neurogenesis (Duman et al, 2001). Since these initial observations, adult hippocampal neurogenesis has been shown to be required for some, but not all, of the behavioral effects of antidepressants (David et al, 2009; Santarelli et al, 2003). However, one important remaining question is whether increasing levels of adult hippocampal neurogenesis is sufficient for antidepressant-like effects on behavior. Tangential support for this hypothesis includes a study in which deletion of neurofibromin 1 from adult-born cells increased levels of adult neurogenesis and had an antidepressant-like effect on behavior; however, this manipulation not only increased the number of adult-born neurons, but also affected these cells in other ways, such as by activating ERK signaling (Li et al, 2012). More recently, the P7C3 compound has been shown to increase adult neurogenesis and has an antidepressant-like effect on social interaction behavior following social defeat; however, P7C3 likely exerts its effects through multiple biological processes including, but not limited to, neurogenesis (Walker et al, 2014; Wang et al, 2014; Yin et al, 2014).

To directly assess the effects of selectively increasing the number of adult-born neurons, we previously generated a transgenic mouse line with increased adult hippocampal neurogenesis (Sahay et al, 2011). Under baseline conditions, increased adult neurogenesis enhances pattern separation, but does not impact anxiety or depression-related behavior (Sahay et al, 2011). Here, we show that increasing adult hippocampal neurogenesis using this same transgenic mouse model is sufficient to promote resilience to chronic corticosterone (CORT) administration, a model of stress. These findings extend our understanding of the contribution of adult neurogenesis to anxiety and depression, and suggest that neurogenesis may be harnessed to develop novel antidepressants.

MATERIALS AND METHODS

Mice

iBax mice are homozygous for a loxP-flanked Bax allele (Takeuchi et al, 2005), hemizygous for a Nestin-CreERT2 transgene (Dranovsky et al, 2011), and maintained on a mixed C57BL/6 and 129/SvEv background, as previously described (Sahay et al, 2011). Although some ectopic expression has been reported in this transgenic line (Sun et al, 2014), it is unlikely to influence our phenotype since these ectopic cells are not undergoing apoptosis, and are therefore unlikely to be affected by the Bax deletion. Mice were housed two to five per cage and maintained on a 12 h light/dark schedule with continuous access to food and water. All behavioral testing was conducted during the light cycle with approval from the Institutional Animal Care and Use Committees at both Columbia University and the New York State Psychiatric Institute. All mice used in experiments reported here are males.

Drug Administration

Tamoxifen (TAM) was dissolved in a solution of corn oil (C8267, Sigma, St Louis, MO) and 10% ethanol. iBax mice (8–10-week-old) received 2 mg TAM (10 mg/ml, Sigma), or the same volume of corn oil and ethanol (vehicle), intraperitoneally once per day for 5 consecutive days. This dose of TAM has previously been shown to have no effect on behavior in iBax mice (Sahay et al, 2011).

CORT (C2505, Sigma) was dissolved in .45% beta-cyclodextrin, administered ad libitum in opaque bottles, and replaced every 3–4 days. iBax mice received 70 μg/ml CORT, equivalent to 10 mg/kg/day, an effective dose in this strain (unpublished data).

Bromodeoxyuridine (BrdU) (150 mg/kg body weight, dissolved in 0.9% NaCl (Roche, Indianapolis, IN)) was injected intraperitoneally once per day for 2 days at designated time points.

Behavioral Testing

Mice were tested in the open field (MED Associates, St Albans, VT), a square 43.2 × 43.2 cm enclosure with infrared beams to detect animal movement, for 30 min. The center region was defined by the inner 21.2 × 21.2 cm area. Total distance, percent center distance (the percent of the total distance that occurs in the center region), time in center, and center entries were analyzed by MED Associates software.

Mice were tested in the elevated plus maze for 5 min. The maze used has arms 7.6-cm wide, 28-cm long, and 31 cm above the floor. Two opposing closed arms have 15 cm walls, while two opposing open arms have a 1 cm lip. Testing was video recorded and later analyzed by an observer (blind to genotype) for time spent and entries into the open arms.

In the tail suspension test, mice were suspended from a table-top by their tails for 5 min. This test was video recorded and mobility was analyzed using ViewPoint Life Sciences software (ViewPoint, Montréal, Canada).

The forced swim test was conducted as in the study by Sahay et al (2011) for 6 min on each of 2 days, with the first day serving the purpose of pre-exposure. Mobility on the second day was analyzed using ViewPoint Life Sciences Software.

In preparation for the novelty-suppressed feeding test, mice were food deprived in their home cages for 24–26 h before testing. The testing apparatus consisted of a plastic arena (45-cm long, 15-cm high, and 30 cm wide) with the floor covered by 2 cm of wood-chip bedding. A single food pellet (familiar laboratory mouse chow) was placed on circular white filter paper (12 cm in diameter) in the center of the arena. Mice were placed in a corner of the arena, and the latency to begin feeding on the food pellet was recorded (up to a maximum time of 10 min). Testing was conducted under bright light conditions.

Plasma CORT Levels

In the no-stress experiment (Figure 2), hypothalamic–pituitary–adrenal (HPA) axis activity was assessed in two paradigms. In the first, mice were placed in a decapicone plastic restraining device (Braintree Scientific, Braintree, MA) for 30 min, and then placed in a novel cage (as in the study by Snyder et al (2011)). Blood was taken from different groups of mice for each time point: from the home cage (before restraint), immediately after restraint, or after 30 or 60 min in a novel cage following restraint. Blood was collected via submandibular bleed using a Goldenrod Animal Lancet (MEDIpoint, Mineola, NY), into a tube containing EDTA. Blood was centrifuged at 2000 rpm for 5 min for separation of plasma, which was stored at −20 °C until assayed using an EIA kit (Arbor Assays, Ann Arbor, MI).

In the second paradigm, mice were placed in a novel cage without bedding for 15 min (as in the study by Schloesser et al (2009)). Blood was collected immediately afterwards, and treated as described above.

To examine HPA axis response to acute stress in the chronic CORT model (Figure 4e), mice were subjected to a 1 min swim stress, where animals were placed in a beaker of room temperature water. Trunk blood was collected 5 min later (as in the study by David et al (2009)) and treated as above.

Immunohistochemistry

At sacrifice, mice were anesthetized with ketamine and xylazine (100 mg/kg and 7 mg/kg, respectively) and transcardially perfused with cold saline and 4% paraformaldehyde. Brains were postfixed in 4% paraformaldehyde overnight, transferred to 30% sucrose for cryoprotection, and coronally sectioned (35 μm).

For BrdU, immunostaining was conducted on slides as in the study by Sahay et al (2011) with rat anti-BrdU (OBT0030 Serotec, Raleigh, NC) and donkey anti-rat Cy3 (Jackson ImmunoResearch, Westgrove, PA) antibodies. Analysis was conducted using an Axiovert 200 microscope (Zeiss, Oberkochen, Germany) by an investigator blind to treatment.

Doublecortin (DCX) immunostaining was conducted as in the study by Sahay et al (2011) with goat anti-DCX (SC-8066, Santa Cruz Biotechnology, Santa Cruz, CA) and biotin donkey anti-goat (Jackson ImmunoResearch) antibodies, followed by avidin/biotin (ABC kit, Vector Laboratories, Burlingame, CA), and DAB peroxidase (Vector Laboratories). An investigator blind to treatment counted every sixth section throughout the dentate gyrus for the total number of DCX-positive neurons and for those with tertiary dendrites. Analysis was conducted using an Axio Observer A.1 microscope (Zeiss).

Statistical Methods

Due to animal number constraints, we were unable to test all four groups (control, TAM, CORT, and TAM+CORT) concurrently, and therefore instead tested vehicle and TAM in one experiment, and vehicle, CORT, and TAM+CORT in a second experiment. One-way ANOVAs were used to assess significance of comparisons between vehicle and TAM in the first experiment, and between vehicle and CORT as well as between CORT and TAM+CORT in the second experiment, which were the comparisons we were interested in a priori. To statistically assess main effects of TAM and CORT, or interactions between the two variables, we combined and normalized data from these two experiments, as depicted in Supplementary Figure 1. To do this, for each behavioral measure, controls from each test were normalized to a 0–10 scale using unity base normalization, and then the other groups in each experiment were normalized accordingly. The following equation was used: [a+(x_−_A)(b_−_a)]/(B_−_A) where _A_=minimum value in the control group, _B_=maximum value in the control group, _a_=0 (normalized minimum), _b_=10 (normalized maximum), and _x_=the data point to be normalized.

To analyze the effects of two variables in the novelty suppressed feeding test, data were analyzed using the Cox Proportional Hazard model, using SAS software (SAS Institute, Cary, NC). The effects of single variables in this test were analyzed using Kaplan–Meier Survival Analysis. All other statistical analyses were conducted using StatView software (SAS Institute). Results were considered statistically significant if P<0.05.

Extended statistical details for all experiments are included in Supplementary Table 1.

RESULTS

Genetic Deletion of Bax Increases Adult Hippocampal Neurogenesis, but does not Affect Anxiety and Antidepressant-Responsive Behavior Under No-Stress Conditions

To genetically increase levels of adult neurogenesis, we utilized a bi-transgenic mouse model that expresses the TAM-dependent recombinase CreERT2 under control of the Nestin promoter, as well as floxed alleles of the pro-apoptotic gene Bax. In this mouse line, TAM treatment allows inducible deletion of Bax in neural stem cells and their progeny (iBax mice). Normally, 60–80% of adult-born dentate granule neurons undergo cell death before reaching maturity through a process that requires BAX (Dayer et al, 2003). This transgenic design increases neurogenesis by preventing cell death in the adult-born neuronal population (Sahay et al, 2011) (Figure 1a).

Figure 1

figure 1

In the absence of stress, genetically increasing adult hippocampal neurogenesis in iBax mice increases adult hippocampal neurogenesis, but does not affect anxiety- and antidepressant-responsive behavior. (a) Schematic: vehicle-treated animals have normal levels of adult hippocampal neurogenesis where stem cells (red) can undergo symmetric or asymmetric division to produce mature granule cells (purple), however, the majority of produced cells undergo BAX-dependent cell death during maturation. Tamoxifen treatment in iBax mice prevents BAX-mediated cell death in recombined cells, leading to an increased number of adult-born granule cells. (b) Experimental timeline: iBax mice were injected with either vehicle (top line) or tamoxifen (TAM; bottom line) 6 weeks before behavioral testing. (c) TAM increases the total number of BrdU-positive cells in the dentate gyrus (_P_=0.007). Significant differences are seen both in the dorsal (_P_=0.004) and ventral (_P_=0.017) subregions. The majority of BrdU-labeled cells are colabeled with the mature neuronal marker NeuN, and this percentage does not differ between groups when assessed throughout the whole, dorsal, or ventral hippocampus (_P_>0.05). (d) TAM has no effect on the number of BrdU-positive cells observed in the arcuate nucleus of the hypothalamus (_P_>0.05). (e and f) Mice treated with vehicle or TAM show similar locomotion in the open field test, as assessed by total distance (_P_>0.05). Both groups also show anxiety-like behavior in the open field test, as assessed by percent center distance (_P_>0.05), and in the elevated plus maze, as assessed by time spent in the open arms (_P_>0.05) or open arm entries (_P_>0.05). (g) No differences were observed between mice treated with vehicle or TAM in depression-like behavior as observed by mobility in the tail suspension test (_P_>0.05). In the line graph, data is represented in 1-min bins for the duration of the test. All error bars represent SEM. *P<0.05, **P<0.01. BrdU, bromodeoxyuridine; NeuN, neuronal nuclei; TAM, Tamoxifen.

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Changes in neurogenesis were assessed after sacrifice by the number of cells labeled with the thymidine-analog BrdU, which was injected the week following TAM induction, and becomes incorporated into cells that are dividing at the time of injection (Figure 1b). As previously reported (Sahay et al, 2011), we observed a large increase in the number of BrdU-labeled cells in the dentate gyrus in mice treated with TAM as compared with vehicle. Here, at 8 weeks post-BrdU injection, we observed a fourfold increase in the total number of BrdU-positive cells (F(1,8)=12.844, P<0.01) (Figure 1c), an increase seen in both dorsal (3.7-fold increase, F(1,8)=16.595, P<0.01), and ventral (4.5-fold increase, F(1,8)=8.968, P<0.05) dentate gyrus.

While neurons make up the majority of adult-born cells labeled with BrdU in the hippocampus, stem cells, and astrocytes and potentially even oligodendrocytes (Chetty et al, 2014) are also found. To determine the proportion of neurons produced, colabeling was assessed between BrdU and the mature neuronal marker neuronal nuclei (NeuN). We observed no significant differences between groups in the proportion of BrdU labeled cells that were colabeled with NeuN, when assessed throughout the entire dentate gyrus, or in either the dorsal or ventral subregions (F(1,4)<1.056, _P_>0.05) (Figure 1c).

Recently, studies have suggested the presence of adult-neurogenesis in the hypothalamus (Batailler et al, 2014; Saaltink et al, 2012), involved in maintaining weight and metabolism (Lee and Blackshaw, 2012). To determine whether hypothalamic adult neurogenesis is affected in iBax mice, we performed analysis of the number of BrdU-labeled cells in the arcuate nucleus, a portion of the hypothalamus where the presence of adult-born cells has been reported (Kokoeva et al, 2005; Pierce and Xu, 2010; Xu et al, 2005). We observed no difference in the numbers of BrdU-labeled cells between vehicle- and TAM-treated mice (F(1,8)=1.694, _P_>0.05) (Figure 1d).

Initial characterization of this mouse model showed that under baseline, no-stress conditions, increasing adult neurogenesis enhances pattern separation, but has no effect on anxiety or depression-related behavior (Sahay et al, 2011). To confirm and extend this finding with additional behavioral tests, iBax mice were treated with either vehicle or TAM, and tested for anxiety and depression-related behavior 6 weeks later (Figure 1b). As expected, we observed no significant differences between vehicle- and TAM-treated groups in the open field, elevated plus maze, or tail suspension tests (Fs<3.401, _P_>0.05) (Figure 1e–g).

Increasing Adult Hippocampal Neurogenesis does not Affect HPA Axis Response to Acute Stress

Elimination of adult-hippocampal neurogenesis has been shown to impair regulation of the HPA axis, as several studies have now shown that mice with ablated adult neurogenesis have a heightened HPA axis response to acute stress (Schloesser et al, 2009; Snyder et al, 2011; Surget et al, 2011). We therefore initially hypothesized that increasing adult hippocampal neurogenesis might improve HPA axis regulation, which we expected to observe as decreased plasma CORT following acute stress. We tested whether this process is affected by increased adult neurogenesis, using the same paradigms which have been shown to require adult hippocampal neurogenesis for proper HPA axis regulation. Here, we observed no differences in plasma CORT between vehicle and TAM groups at baseline, following restraint stress, or following the mild stress of a novel cage (Fs<2.154, _P_>0.05) (Figure 2). Together, these results suggest that at baseline, increasing adult hippocampal neurogenesis is not sufficient to affect anxiety and depression-related behaviors or HPA axis regulation.

Figure 2

figure 2

Genetically increasing adult hippocampal neurogenesis does not affect the HPA axis response to acute stress. (a) Experimental timeline. (b and c) There were no differences between vehicle and TAM groups in plasma CORT levels before or at various time points following 30 min of restraint stress or immediately following 15 min in a novel cage (_P_>0.05). _n_=4–11/group. All error bars represent SEM. CORT, corticosterone; HPA, hypothalamic–pituitary–adrenal; TAM, Tamoxifen.

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Genetic Deletion of Bax in Adult Neural Stem Cells Prevents the Effects of Chronic CORT on Adult Neurogenesis

It has been repeatedly shown that ablation of adult neurogenesis has no effect on anxiety- or depression-related behavior at baseline, but can prevent the enhancing effects of antidepressants and enriched environments (Santarelli et al, 2003; Schloesser et al, 2010). In addition, in some strains of mice, antidepressants have no effect at baseline, but do rescue the effects of chronic unpredictable mild stress (Surget et al, 2009) or chronic CORT (David et al, 2009). We therefore reasoned that even though increasing adult neurogenesis shows no effect on behavior at baseline, it might prevent the effects of stress. To test this hypothesis, we subjected iBax mice to chronic CORT (Figure 3a).

Figure 3

figure 3

Genetic ablation of Bax in neural stem cells and progenitors protects against chronic CORT-induced reduction in adult hippocampal neurogenesis. (a) Experimental timeline: iBax mice were injected with either vehicle or TAM. Six weeks later, mice received vehicle or CORT via drinking water for the remainder of the experiment. Three behavioral groups were tested, vehicle (top line), CORT alone (middle line), and TAM+CORT (bottom line). (b and c) Representative images of BrdU and DCX in the dentate gyrus (scale bars 100 um). (d) While no significant difference in the number of BrdU-positive cells is observed between vehicle- and CORT-treated mice (_P_>0.05), TAM+CORT treatment increases the number of BrdU-positive cells (_P_=0.046) (dorsal _P_=0.064; ventral _P_=0.027). The majority of BrdU-labeled cells are colabeled with the mature neuronal marker NeuN. The percent of BrdU-labeled cells colabeled with NeuN is the same between all groups in the dorsal dentate gyrus. In the ventral dentate gyrus, a lower percentage of BrdU-positive cells are colabeled with NeuN in CORT-treated mice, compared with those treated with TAM+CORT (_P_=0.022). (e) CORT treatment decreases the total number of DCX-positive neurons (_P_=0.005), and there is a trend for a decrease in the number of DCX-positive neurons with tertiary dendrites (_P_=0.07). TAM+CORT prevents these effects (_P_=0.002 for total DCX-positive neurons; _P_=0.002 for DCX-positive neurons with tertiary dendrites). In the dorsal and ventral subregions, the total number of DCX-positive neurons is decreased by CORT (dorsal _P_=0.07; ventral _P_=0.039), and rescued in TAM+CORT-treated mice (dorsal _P_=0.028; ventral _P_=0.002); the number of DCX-positive neurons with tertiary dendrites is increased in TAM+CORT-treated mice compared with mice treated with CORT alone (dorsal _P_=0.013; ventral _P_=0.001). _n_=4–7/group in all analyses. All error bars represent SEM. *P<0.05, **P<0.01. BrdU, bromodeoxyuridine; CORT, corticosterone; DCX, doublecortin; NeuN, neuronal nuclei; TAM, Tamoxifen.

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To determine the impact of genetic deletion of Bax on neurogenesis in mice treated with chronic CORT, we used immunohistochemistry. As in the previous experiment, mice were injected with BrdU the week following TAM induction. Here we observed no significant difference between vehicle- and CORT-treated mice (F(1,6)=1.086, _P_>0.05) (Figure 3d). This was expected, as cells labeled at the time of BrdU injection were 4-weeks old at the onset of CORT treatment (see timeline Figure 3a), and therefore their number was unaffected by CORT. However, TAM+CORT animals had significantly greater numbers of BrdU-labelled cells compared with CORT alone (168.5% increase, F(1,9)=5.33, P<0.05) (Figure 3d), with similar effects in both dorsal and ventral dentate gyrus subregions.

Colabeling with NeuN was then used to determine the proportion of BrdU-labeled neurons in each group. While no differences were observed in the dorsal subregion, in the ventral dentate gyrus, TAM+CORT-treated animals were found to have a significantly higher percentage of cells colabeled for BrdU and NeuN than animals treated with CORT alone (F(1,8)=7.987, P<0.05) (Figure 3d).

To assess neurogenesis levels during behavioral testing, immunostaining was performed for the immature neuronal marker DCX, which labels cells that are a few days to about 3 weeks of age before sacrifice (Brown et al, 2003). We found that CORT treatment significantly decreased the number of DCX-positive cells compared with vehicle (24% decrease, F(1,7)=15.994, P<0.01) (Figure 3e), while this was increased in the TAM+CORT group (69% increase compared with CORT-alone group, F(1,10)=16.789, P<0.01). We next assessed the number of DCX-positive neurons with tertiary dendrites, which represent a more mature subset of adult-born granule cells. Here, there was a trend for an effect of CORT (31% decrease, F(1,7)=4.754, _P_=0.07), and a significant increase in TAM+CORT-treated mice (120% increase compared with CORT-alone group, F(1,10)=18.025, P<0.01). When the data was split based on dorsal and ventral subregions, similar effects were seen in both subregions.

Increased Adult Hippocampal Neurogenesis Promotes Resilience to the Behavioral Effects of Chronic CORT Administration, but Does Not Alter HPA Axis Activity

After 4 weeks of CORT administration, mice were assessed on various behavioral tests of anxiety-related and antidepressant-responsive behavior. In some tests, there were no differences between any groups. For example, in the open field test, all groups behaved similarly in total distance, a measure of locomotive activity, as well as percent center distance, an anxiety measure (Fs<1.755, _P_>0.05) (Figure 4b). However, the subset of behavioral tests that were affected by CORT, were also affected by an increase in neurogenesis (TAM+CORT group). In the elevated plus maze, CORT had an anxiogenic effect, manifested by decreased time spent in the open arms (F(1,25)=6.86, P<0.05) as well as fewer open arm entries (F(1,25)=6.547, P<0.05) (Figure 4c), while this was reversed in TAM+CORT-treated mice (F(1,28)=6.273, P<0.05 for open arm time, F(1,28)=4.252, P<0.05 for open arm entries), showing that increasing adult hippocampal neurogenesis is sufficient to produce an anxiolytic effect in this test. In the tail suspension test, CORT decreased mobility as compared with vehicle (F(1,26)=5.13, P<0.05) (Figure 4d), and TAM+CORT treatment increased mobility (F(1,26)=9.597, P<0.01), providing evidence that increased adult hippocampal neurogenesis is sufficient to promote stress resilience in this antidepressant-responsive test.

Figure 4

figure 4

Genetically increasing adult hippocampal neurogenesis in iBax mice prevents the effects of chronic CORT on mood-related behavior, but does not affect HPA axis regulation. (a) Experimental timeline. (b) No statistically significant differences were seen between groups in total distance or percent center distance in the open field test (_P_>0.05). _n_=8–10/group. (c) In the elevated plus maze, CORT-treated mice spent significantly less time in the open arms (_P_=0.015) and had fewer open arm entries (_P_=0.017) than controls. These effects were reversed in TAM+CORT-treated mice (_P_=0.018 for open arm time, _P_=0.049 for open arm entries). _n_=12–15/group. (d) In the tail suspension test, CORT-treated mice displayed decreased mobility (_P_=0.032), which was reversed in TAM+CORT-treated mice (_P_=0.005). In the line graph, data is represented in 1-min bins for the duration of the test. _n_=14–15/group. (e) Trunk blood was collected 5 min after a 1-min swim stress, from which plasma was isolated. There is a strong trend for CORT-treated mice to have lower plasma CORT levels than controls following forced swim stress (_P_=0.05), but no difference between CORT and TAM+CORT groups (_P_=0.91). _n_=5–6 group. All error bars represent SEM. *P<0.05, **P<0.01. CORT, corticosterone; HPA, hypothalamic–pituitary–adrenal; TAM, Tamoxifen.

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Along with the open field test, the forced swim and novelty suppressed feeding tests also showed no effect of CORT and a lack of an effect for TAM treatment (Fs<2.419, _P_>0.05) (Supplementary Figure 1d and e). Consistent with the lack of an effect of increasing adult neurogenesis in the baseline, no-stress group, this suggests that neurogenesis may specifically affect behaviors that are impacted by chronic CORT in this paradigm.

Next, we were interested in assessing HPA axis regulation in these mice, to determine whether increasing neurogenesis prevents the effects of chronic CORT administration on HPA axis regulation. We have previously observed that our model of chronic CORT treatment has no effect on baseline CORT levels (unpublished data), however, does diminish the elevated levels of CORT produced in response to acute swim stress, as compared with control animals (David et al, 2009). Here, using the same paradigm, we observed a strong trend for decreased CORT levels following this acute swim stress in CORT-treated mice compared with controls (F(1,8)=5.155, _P_=0.05) (Figure 4e), and no difference between CORT and TAM+CORT-treated mice (F(1,9)=0.016, _P_>0.05), suggesting that both of these groups have similar impairment of the endogenous HPA axis response to acute stress. In the context of chronic CORT, we therefore observe a dissociation, where increased neurogenesis affects anxiety- and depression-related behavior, but not HPA axis regulation.

DISCUSSION

Here we have shown that increasing adult hippocampal neurogenesis is sufficient to promote resilience to chronic CORT administration. In iBax mice, we genetically block cell death in adult-born cells from neural precursors, which increases the number of adult-born neurons that reach maturation. In this way, we specifically mimicked this single property of antidepressants to increase adult neurogenesis, and showed that it is sufficient for antidepressant-like behavioral effects in tests that are sensitive to chronic CORT.

We show that chronic CORT has anxiogenic and depressive effects in a subset of behavioral tests, the elevated plus maze and the tail suspension test, which are prevented in mice with increased adult hippocampal neurogenesis (Figure 4). Conversely, in tests in which CORT has no effect, such as the open field test, increasing neurogenesis also has no effect. In this case, the behavior of all groups is similar to controls, and mimics the lack of an effect under baseline, no-stress conditions (Figure 1). The disparity between the effects in these two situations, baseline and chronic CORT, suggests that in iBax mice, increased neurogenesis only affects anxiety and depression-related behavior when animals are in a stressed-like state.

Chronic CORT administration was used as a model of stress in these studies. While prior studies have observed effects of chronic CORT administration in different tests than observed here (David et al, 2009), we note that different strains of mice often display inconsistent behavioral phenotypes and responses to various stress models and drugs (Dulawa et al, 2004; Razzoli et al, 2011; Yalcin et al, 2008). In addition, as stress has many effects beyond CORT, which are not modelled here, additional studies would be necessary to fully understand how increased adult hippocampal neurogenesis interacts with stress.

A recent study found that BAX is required for long-term depression (LTD) but not basal synaptic transmission or long-term potentiation (Jiao and Li, 2011). Although the role of BAX in LTD was not examined in dentate granule neurons in that study, if this function is conserved across cell-types, including maturing adult-born neurons, then one cannot rule out the possibility that decreased LTD of adult-born neurons may contribute to some of the behavioral effects observed in this study. This is, however, unlikely, since the electrophysiological and behavioral impact of young neurons in iBax mice is consistent with increased neurogenesis rather than with altered properties (Sahay et al, 2011). Another potential concern is that the Nestin-CreERT2 driver line used here has been shown to drive ectopic expression of reporter lines (Sun et al, 2014), raising the possibility that LTD may be affected in additional cells of iBax mice. However, we have conducted additional studies utilizing the same Nestin-CreERT2 line to target adult-born neurons, where we see that x-irradiation prevents the electrophysiological impact of these cells on hippocampal circuits, ruling out the possibility that ectopic expression through this driver line affects hippocampal activity (unpublished results). Nevertheless, we cannot rule out at present the possibility of ectopic effects in other brain regions.

One prevailing hypothesis in the literature is that adult neurogenesis affects anxiety- and depression-related behaviors through altered regulation of the HPA axis, since the absence of adult neurogenesis impairs HPA axis regulation following acute stress (Schloesser et al, 2009; Snyder et al, 2011) and prevents the effects of antidepressants on the HPA axis (Surget et al, 2011). While these studies suggest that intact adult hippocampal neurogenesis is required for proper HPA axis activity, our data suggest that increasing adult hippocampal neurogenesis is not sufficient to affect this process (Figures 2 and 4e). While our findings do not fit into the framework laid out by the ablation studies cited above, our study is the first to test the HPA axis response using a model of increased, rather than decreased, adult hippocampal neurogenesis. Together, it appears that a certain level of neurogenesis is required for proper HPA axis regulation, but that levels above this threshold have no additive effect. While it is possible that increased neurogenesis may have more subtle effects on the HPA axis that were not observed here, our data suggest that increased neurogenesis likely can affect behavior through a mechanism independent of HPA axis regulation. Nevertheless, we do note that under different stress conditions, where the endogenous HPA axis is active, increased neurogenesis could affect behavior through an additional mechanism involving the HPA axis.

A previous study showed that the effects of social defeat stress and environmental enrichment on both neurogenesis and stress-related behavior are dependent on modulation of the HPA axis, as adrenalectomized mice with clamped CORT levels were unaffected by these environmental manipulations (Lehmann et al, 2013). Data presented here further suggests that CORT-mediated changes in neurogenesis are required for behavioral effects, as mice with increased neurogenesis do not display CORT-induced behavioral effects.

A recent study has shown that acute CORT treatment (for 7 days) shifts the balance of cell types produced towards fewer neurons and more oligodendrocytes, and has suggested the possibility that this shift may underlie behavioral effects of CORT treatment (Chetty et al, 2014). We note that here we did not observe a significant effect of CORT treatment on the percent of BrdU-positive cells that are colabeled with the neuronal marker NeuN (Figure 3d), possibly because BrdU was injected 4 weeks before the onset of CORT treatment (Figure 3a). However, we did observe a significant difference between CORT and CORT+TAM groups in the proportion of BrdU-positive cells colabeled with NeuN. This suggests that these two groups differ not only in the number of adult-born cells, but also in cell-types and functions.

It is particularly interesting that this difference is observed specifically in the ventral, but not dorsal, dentate gyrus. Numerous studies have now shown that the dorsal and ventral hippocampus have different gene expression, connectivity, and function (Fanselow and Dong, 2010; Kheirbek et al, 2013). In addition, many studies have observed larger effects of chronic stressors and antidepressants on ventral than dorsal neurogenesis (Tanti and Belzung, 2013b; Wu and Hen, 2014a; Wu et al, 2014b). Although we do not see large differences in neurogenesis levels between dorsal and ventral dentate gyrus, observation of this difference in proportion of adult-born neurons produced in the ventral dentate gyrus supports the hypothesis that neurogenesis in the ventral hippocampus may be particularly important for anxiety- and depression-related behavior.

While one identified function of the ventral hippocampus is to provide negative feedback to the HPA axis, primarily via connectivity through the bed nucleus of the stria terminalis (Cullinan et al, 1993; Herman et al, 1992), our data suggests that this process is not impaired by increasing adult hippocampal neurogenesis. We therefore suggest that the ventral hippocampus may mediate the behavioral effects observed here through a different mechanism, possibly through connectivity to other brain structures which receive ventral hippocampal input, such as the amygdala or nucleus accumbens (Canteras and Swanson, 1992; Fanselow and Dong, 2010; Namura et al, 1994)

While the studies presented here suggest that increasing adult hippocampal neurogenesis can protect against the behavioral effects of chronic exogenous CORT administration, it remains to be seen whether this manipulation can rescue behavior if neurogenesis is increased after chronic CORT. As antidepressants are generally prescribed after the onset of depression, this is an important point to be addressed for the translational feasibility of targeting neurogenesis. In addition, imaging tools being developed to detect levels of adult hippocampal neurogenesis in depressed patients may allow increased adult hippocampal neurogenesis to serve as a biomarker for antidepressant efficacy (Couillard-Despres and Aigner, 2011; Tanti and Belzung, 2013a).

Neural stem cells in the adult mammalian brain provide a resource that may be harnessed to treat various disorders. The experiments presented here suggest that expanding the production of adult-born neurons may be beneficial for the treatment of depression and anxiety disorders.

FUNDING AND DISCLOSURE

RH is supported by a US National Institute of Health grant R37MH068542, the National Institute of Aging grant R01AG043688, the Hope for Depression Research Foundation (HDRF), and the New York State Stem Cell Science (NYSTEM) contract CO26430. RH also receives compensation as a consultant for Lundbeck and Servier. AS is supported by a US National Institutes of Health grant 1-R01MH104175, the Ellison Medical Foundation New Scholar in Aging, and a Whitehall Foundation grant. AS is also a consultant for Psybrain LLC. ASH declares no conflict of interest.

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Acknowledgements

We would like to thank K. Keener, M. Lauring, and C. Price for technical assistance.

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Authors and Affiliations

  1. Department of Neuroscience, Columbia University, New York, NY, USA
    Alexis S Hill & René Hen
  2. Department of Psychiatry, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA
    Amar Sahay
  3. Harvard Stem Cell Institute, Boston, MA, USA
    Amar Sahay
  4. Harvard Medical School, Boston, MA, USA
    Amar Sahay
  5. Department of Psychiatry, Columbia University, New York, NY, USA
    René Hen
  6. Department of Pharmacology, Columbia University, New York, NY, USA
    René Hen
  7. Division of Integrative Neuroscience, The New York State Psychiatric Institute, New York, NY, USA
    René Hen

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  1. Alexis S Hill
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  2. Amar Sahay
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  3. René Hen
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Correspondence toAmar Sahay or René Hen.

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Hill, A., Sahay, A. & Hen, R. Increasing Adult Hippocampal Neurogenesis is Sufficient to Reduce Anxiety and Depression-Like Behaviors.Neuropsychopharmacol 40, 2368–2378 (2015). https://doi.org/10.1038/npp.2015.85

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