Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation (original) (raw)
Huch, M. & Koo, B.K. Modeling mouse and human development using organoid cultures. Development142, 3113–3125 (2015). ArticleCAS Google Scholar
Eiraku, M. et al. Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature472, 51–56 (2011). ArticleCAS Google Scholar
Lancaster, M.A. et al. Cerebral organoids model human brain development and microcephaly. Nature501, 373–379 (2013). ArticleCAS Google Scholar
McCracken, K.W., Howell, J.C., Wells, J.M. & Spence, J.R. Generating human intestinal tissue from pluripotent stem cells. Nat. Protoc.6, 1920–1928 (2011). ArticleCAS Google Scholar
McCracken, K.W. et al. Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature516, 400–404 (2014). ArticleCAS Google Scholar
Sato, T. et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature459, 262–265 (2009). ArticleCAS Google Scholar
Barker, N. et al. Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units. Cell Stem Cell6, 25–36 (2010). ArticleCAS Google Scholar
Huch, M. et al. In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature494, 247–250 (2013). ArticleCAS Google Scholar
Huch, M. et al. Long-term culture of genome-stable bipotent stem cells from adult human liver. Cell160, 299–312 (2015). ArticleCAS Google Scholar
Huch, M. et al. Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis. EMBO J.32, 2708–2721 (2013). ArticleCAS Google Scholar
Boj, S.F. et al. Organoid models of human and mouse ductal pancreatic cancer. Cell160, 324–338 (2015). ArticleCAS Google Scholar
Koo, B.K. et al. Controlled gene expression in primary Lgr5 organoid cultures. Nat. Methods9, 81–83 (2012). ArticleCAS Google Scholar
Schwank, G. et al. Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. Cell Stem Cell13, 653–658 (2013). ArticleCAS Google Scholar
Zaret, K.S. Genetic programming of liver and pancreas progenitors: lessons for stem-cell differentiation. Nat. Rev. Genet.9, 329–340 (2008). ArticleCAS Google Scholar
Duncan, A.W., Dorrell, C. & Grompe, M. Stem cells and liver regeneration. Gastroenterology137, 466–481 (2009). Article Google Scholar
Zhao, R. & Duncan, S.A. Embryonic development of the liver. Hepatology41, 956–967 (2005). ArticleCAS Google Scholar
Zorn, A.M. Liver development. In StemBook 31 Oct 2008 (ed. Harvard Stem Cell Institute, Cambridge, MA, 2008).
Shih, H.P., Wang, A. & Sander, M. Pancreas organogenesis: from lineage determination to morphogenesis. Annu. Rev. Cell Dev. Biol.29, 81–105 (2013). ArticleCAS Google Scholar
Michalopoulos, G.K. et al. Histological organization in hepatocyte organoid cultures. Am. J. Pathol.159, 1877–1887 (2001). ArticleCAS Google Scholar
Greggio, C. et al. Artificial three-dimensional niches deconstruct pancreas development. Development140, 4452–4462 (2013). ArticleCAS Google Scholar
D'Amour, K.A. et al. Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat. Biotechnol.24, 1392–1401 (2006). ArticleCAS Google Scholar
Pagliuca, F.W. et al. Generation of functional human pancreatic β cells. Cell159, 428–439 (2014). ArticleCAS Google Scholar
Zaret, K.S. & Grompe, M. Generation and regeneration of cells of the liver and pancreas. Science322, 1490–1494 (2008). ArticleCAS Google Scholar
Bort, R., Signore, M., Tremblay, K., Martinez Barbera, J.P. & Zaret, K.S. Hex homeobox gene controls the transition of the endoderm to a pseudostratified, cell emergent epithelium for liver bud development. Dev. Biol.290, 44–56 (2006). ArticleCAS Google Scholar
Francis, H. et al. cAMP stimulates the secretory and proliferative capacity of the rat intrahepatic biliary epithelium through changes in the PKA/Src/MEK/ERK1/2 pathway. J. Hepatol.41, 528–537 (2004). ArticleCAS Google Scholar
McCright, B., Lozier, J. & Gridley, T. A mouse model of Alagille syndrome: Notch2 as a genetic modifier of Jag1 haploinsufficiency. Development129, 1075–1082 (2002). CASPubMed Google Scholar
Ahlgren, U., Jonsson, J. & Edlund, H. The morphogenesis of the pancreatic mesenchyme is uncoupled from that of the pancreatic epithelium in IPF1/PDX1-deficient mice. Development122, 1409–1416 (1996). CASPubMed Google Scholar
Donehower, L.A. et al. Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours. Nature356, 215–221 (1992). ArticleCAS Google Scholar
Baker, S.J. et al. Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science244, 217–221 (1989). ArticleCAS Google Scholar
Ingalls, A.M., Dickie, M.M. & Snell, G.D. Obese, a new mutation in the house mouse. J. Hered.41, 317–318 (1950). ArticleCAS Google Scholar
Evers, B. & Jonkers, J. Mouse models of BRCA1 and BRCA2 deficiency: past lessons, current understanding and future prospects. Oncogene25, 5885–5897 (2006). ArticleCAS Google Scholar
Andersson-Rolf, A., Fink, J., Mustata, R.C. & Koo, B.K. A video protocol of retroviral infection in primary intestinal organoid culture. J. Vis. Exp. 90 e51765 (2014).
Koo, B.K. et al. Tumour suppressor RNF43 is a stem-cell E3 ligase that induces endocytosis of Wnt receptors. Nature488, 665–669 (2012). ArticleCAS Google Scholar
Davis, H.E., Morgan, J.R. & Yarmush, M.L. Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys. Chem.97, 159–172 (2002). ArticleCAS Google Scholar
Takebe, T. et al. Vascularized and functional human liver from an iPSC-derived organ bud transplant. Nature499, 481–484 (2013). ArticleCAS Google Scholar
Van Lidth de Jeude, J.F., Vermeulen, J.L., Montenegro-Miranda, P.S., Van den Brink, G.R. & Heijmans, J. A protocol for lentiviral transduction and downstream analysis of intestinal organoids. J. Vis. Exp. 98, e52531 (2015).
Wang, N. et al. Adenovirus-mediated efficient gene transfer into cultured three-dimensional organoids. PLoS One9, e93608 (2014). Article Google Scholar
Klaunig, J.E. et al. Mouse liver cell culture. I. Hepatocyte isolation. In Vitro17, 913–925 (1981). ArticleCAS Google Scholar
Korinek, V. et al. Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC−/− colon carcinoma. Science275, 1784–1787 (1997). ArticleCAS Google Scholar
de Lau, W. et al. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature.476, 293–297 (2011). ArticleCAS Google Scholar