The structure of the exocyst subunit Sec6p defines a conserved architecture with diverse roles (original) (raw)

Nature Structural & Molecular Biology volume 13, pages 555–556 (2006)Cite this article

Abstract

The exocyst is a conserved protein complex essential for trafficking secretory vesicles to the plasma membrane. The structure of the C-terminal domain of the exocyst subunit Sec6p reveals multiple helical bundles, which are structurally and topologically similar to Exo70p and the C-terminal domains of Exo84p and Sec15, despite <10% sequence identity. The helical bundles appear to be evolutionarily related molecular scaffolds that have diverged to create functionally distinct exocyst proteins.

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References

  1. Bonifacino, J.S. & Glick, B.S. Cell 116, 153–166 (2004).
    Article CAS Google Scholar
  2. TerBush, D.R., Maurice, T., Roth, D. & Novick, P. EMBO J. 15, 6483–6494 (1996).
    Article CAS Google Scholar
  3. Hsu, S.C. et al. Neuron 17, 1209–1219 (1996).
    Article CAS Google Scholar
  4. Sivaram, M.V., Saporita, J.A., Furgason, M.L., Boettcher, A.J. & Munson, M. Biochemistry 44, 6302–6311 (2005).
    Article CAS Google Scholar
  5. Guo, W., Roth, D., Walch-Solimena, C. & Novick, P. EMBO J. 18, 1071–1080 (1999).
    Article CAS Google Scholar
  6. Vega, I.E. & Hsu, S.C. J. Neurosci. 21, 3839–3848 (2001).
    Article CAS Google Scholar
  7. Matern, H.T., Yeaman, C., Nelson, W.J. & Scheller, R.H. Proc Natl. Acad. Sci. USA 98, 9648–9653 (2001).
    Article CAS Google Scholar
  8. Dong, G., Hutagalung, A.H., Fu, C., Novick, P. & Reinisch, K.M. Nat. Struct. Mol. Biol. 12, 1094–1100 (2005).
    Article CAS Google Scholar
  9. Lamping, E. et al. Gene 361, 57–66 (2005).
    Article CAS Google Scholar
  10. Novick, P., Field, C. & Schekman, R. Cell 21, 205–215 (1980).
    Article CAS Google Scholar
  11. Wu, S., Mehta, S.Q., Pichaud, F., Bellen, H.J. & Quiocho, F.A. Nat. Struct. Mol. Biol. 12, 879–885 (2005).
    Article CAS Google Scholar
  12. Hamburger, Z.A., Hamburger, A.E., West, A.P. & Weis, W.I. J. Mol. Biol. 356, 9–21 (2006).
    Article CAS Google Scholar
  13. Hsu, S.C. et al. Neuron 20, 1111–1122 (1998).
    Article CAS Google Scholar
  14. Whyte, J.R. & Munro, S. J. Cell Sci. 115, 2627–2637 (2002).
    CAS Google Scholar

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Acknowledgements

We are grateful to S. Ryder, W. Royer, W. Kobertz and R. Gilmore for critical reading of this manuscript and discussions. Thanks to the staff at the X25 and X29 beamlines at the National Synchrotron Light Source and to D. Lambright, B. van den Berg, S. Eathiraj and members of the Lambright laboratory for help with structure determination. This work was supported by US National Institutes of Health grant GM068803 to M.M. and an American Heart Association award to M.V.S.S.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, 01605, Massachusetts, USA
    Mylavarapu V S Sivaram, Melonnie L M Furgason, Daniel N Brewer & Mary Munson

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  1. Mylavarapu V S Sivaram
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  2. Melonnie L M Furgason
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  3. Daniel N Brewer
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  4. Mary Munson
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Correspondence toMary Munson.

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Sivaram, M., Furgason, M., Brewer, D. et al. The structure of the exocyst subunit Sec6p defines a conserved architecture with diverse roles.Nat Struct Mol Biol 13, 555–556 (2006). https://doi.org/10.1038/nsmb1096

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