Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis (original) (raw)

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Cell Research volume 13, pages 171–177 (2003)Cite this article

ABSTRACT

Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromosomes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST) fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated and the localization of VCY protein in human testis was determined by immunohistochemistry. In the testis seminiferous epithelium, VCY proteins were highly expressed in nuclei of germ cells. Using propidium iodide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainly localized in the nucleoli of COS7 cells. In addition, the colocalization for VCY and VCX-8r in COS7 cells was also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtained using yeast two-hybrid system. All the information above indicates that VCX/Y protein family might be involved in the regulation of ribosome assembly during spermatogenesis.

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Acknowledgements

We are grateful to Dr. GA. Rappold for providing the VCX-8r cDNA cosmid. This study was supported by grants from the Special Fund for Major State Basic Research Project (No. G1999055901), National Natural Sciences Foundation of China (No. 30240019), National High Technology Research and Technology of China (No. 2001AA221131), and State Ministry of Science and Technology Program (No. 2002BA711 A01).

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Authors and Affiliations

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan 3-tiao, Beijing, 100005, China
    Sheng Wei ZOU, Xiao Dong ZHANG, Shi Ying MIAO, Qi SHENG & Lin Fang WANG
  2. National Research Institute for Family Planing, WHO Collaboration Center for Research in Human Reproduction, 12 Da Hui Si, Beijing, 100081, China
    Jian Chao ZHANG & Shu Dong ZONG

Authors

  1. Sheng Wei ZOU
  2. Jian Chao ZHANG
  3. Xiao Dong ZHANG
  4. Shi Ying MIAO
  5. Shu Dong ZONG
  6. Qi SHENG
  7. Lin Fang WANG

Corresponding author

Correspondence toLin Fang WANG.

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ZOU, S., ZHANG, J., ZHANG, X. et al. Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis.Cell Res 13, 171–177 (2003). https://doi.org/10.1038/sj.cr.7290161

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