Mitotic trafficking of silicon microparticles (original) (raw)
* Corresponding authors
a University of Texas Health Science Center, Department of Nanomedicine and Biomedical Engineering, 1825 Pressler, Suite 537, Houston, TX 77030
E-mail: Mauro.Ferrari@uth.tmc.edu
Tel: +713-500-4444
b University of Texas MD Anderson Cancer Center, Department of Experimental Therapeutics, Unit 422, 1515 Holcombe Blvd., Houston, TX 77030
c Rice University, Department of Bioengineering, Houston, TX 77005
Abstract
Multistage carriers were recently introduced by our laboratory, with the concurrent objectives of co-localized delivery of multiple therapeutic agents, the “theranostic” integration of bioactive moieties with imaging contrast, and the selective, potentially personalized bypassing of the multiplicity of biological barriers that adversely impact biodistribution of vascularly injected particulates. Mesoporous (“nanoporous”) silicon microparticles were selected as primary carriers in multi-stage devices, with targets including vascular endothelia at pathological lesions. The objective of this study was to evaluate biocompatibility of mesoporous silicon microparticles with endothelial cells using _in vitro_assays with an emphasis on microparticle compatibility with mitotic events. We observed that vascular endothelial cells, following internalization of silicon microparticles, maintain cellular integrity, as demonstrated by cellular morphology, viability and intact mitotic trafficking of vesicles bearing silicon microparticles. The presence of gold or iron oxide nanoparticles within the porous matrix did not alter the cellular uptake of particles or the viability of endothelial cells subsequent to engulfment of microparticles. Endothelial cells maintained basal levels of IL-6 and IL-8 release in the presence of silicon microparticles.
This is the first study that demonstrates polarized, ordered partitioning of endosomes based on tracking microparticles. The finding that mitotic sorting of endosomes is unencumbered by the presence of nanoporous silicon microparticles advocates the use of silicon microparticles for biomedical applications.
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Supplementary files
Article information
DOI
https://doi.org/10.1039/B9NR00138G
Article type
Paper
Submitted
17 Jun 2009
Accepted
07 Sep 2009
First published
05 Oct 2009
Download Citation
Nanoscale, 2009,1, 250-259
Permissions
Mitotic trafficking of silicon microparticles
R. E. Serda, S. Ferrati, B. Godin, E. Tasciotti, X. Liu and M. Ferrari,Nanoscale, 2009, 1, 250DOI: 10.1039/B9NR00138G
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