Ex situ evaluation of the composition of protein corona of intravenously injected superparamagnetic nanoparticles in rats (original) (raw)
* Corresponding authors
a Laboratory of Powder Technology, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland
E-mail: heinrich.hofmann@epfl.ch
b Department of Nanotechnology and Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
E-mail: mahmoudi-m@tums.ac.ir
c Division of Pediatric Cardiology, Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305-5101, USA
d Department of Experimental Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands
e Department of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
E-mail: f.rezaee@umcg.nl
f Department of Radiology, University of Geneva and Geneva University Hospital, 1211 Geneva 14, Switzerland
g Department of Oral Biology, Faculty of Oral and Dental Medicine, Cairo University, Egypt
h Department of Gastroenterology and Hepatology, Erasmus Medical Center, Rotterdam, The Netherlands
Abstract
It is now well recognized that the surfaces of nanoparticles (NPs) are coated with biomolecules (e.g., proteins) in a biological medium. Although extensive reports have been published on the protein corona at the surface of NPs in vitro, there are very few on the in vivo protein corona. The main reason for having very poor information regarding the protein corona in vivo is that separation of NPs from the in vivo environment has not been possible by using available techniques. Knowledge of the in vivo protein corona could lead to better understanding and prediction of the fate of NPs in vivo. Here, by using the unique magnetic properties of superparamagnetic iron oxide NPs (SPIONs), NPs were extracted from rat sera after in vivo interaction with the rat's physiological system. More specifically, the in vivo protein coronas of polyvinyl-alcohol-coated SPIONs with various surface charges are defined. The compositions of the corona at the surface of various SPIONs and their effects on the biodistribution of SPIONs were examined and compared with the corona composition of particles incubated for the same time in rat serum.
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Article information
DOI
https://doi.org/10.1039/C4NR02793K
Article type
Paper
Submitted
21 May 2014
Accepted
09 Aug 2014
First published
12 Aug 2014
Download Citation
Nanoscale, 2014,6, 11439-11450
Author version available
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Ex situ evaluation of the composition of protein corona of intravenously injected superparamagnetic nanoparticles in rats
U. Sakulkhu, L. Maurizi, M. Mahmoudi, M. Motazacker, M. Vries, A. Gramoun, M. Ollivier Beuzelin, J. Vallée, F. Rezaee and H. Hofmann,Nanoscale, 2014, 6, 11439DOI: 10.1039/C4NR02793K
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