Enhancement of the antimicrobial activity of cinnamon essential oil-loaded electrospun nanofilm by the incorporation of lysozyme (original) (raw)
* Corresponding authors
a School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
E-mail: bbhwu@scut.edu.cn
Tel: +86-20-22236669
b Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China
Abstract
Essential oils (EOs) are effective antimicrobial agents against a variety of foodborne pathogens; however, their peculiar flavor limits their applications in food preservation. Smaller amounts of EOs in the packaging material are preferable, and a combination of EOs with other antimicrobial compounds can decrease the required dose of EOs while maintaining the appropriate antimicrobial activity. In this study, a novel antimicrobial electrospun nanofilm, namely polyvinyl alcohol/β-cyclodextrin/cinnamon essential oil/lysozyme (PVA/β-CD/CEO/LYS), was fabricated by the combination of CEO and LYS as an antimicrobial agent. The suitable CEO and LYS concentration were determined as 2% (w/w) and 0.25% (w/w), respectively. Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) and thermogravimetric analysis (TGA) indicated the existence of a molecular interaction among PVA, β-CD, CEO, and LYS, which improved the thermal stability of CEO and LYS. Compared to the PVA/LYS and PVA/β-CD/CEO nanofilm with an individual antimicrobial agent, PVA/β-CD/CEO/LYS nanofilm exhibited stronger antibacterial activity against Listeria monocytogenes and Salmonella enteritidis. In addition, it exhibited an excellent antifungal activity against Aspergillus niger and Penicillium. Its minimum inhibition concentration (MIC) against L. monocytogenes and S. enteritidis was approximately 0.8–1 mg mL−1 (corresponding CEO concentration 7.6–9.5 μg mL−1 and LYS concentration 36–45 U mL−1) and minimum bactericidal concentration (MBC) was approximately 6–7 mg mL−1 (corresponding CEO concentration 57–66.5 μg mL−1 and LYS concentration 270–315 U mL−1). Therefore, the antimicrobial PVA/β-CD/CEO/LYS electrospun nanofilm has a potential for application in active food packaging.
- This article is part of the themed collection:Food Packaging
This article is Open Access
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Article information
DOI
https://doi.org/10.1039/C6RA25977D
Article type
Paper
Submitted
28 Oct 2016
Accepted
05 Dec 2016
First published
05 Jan 2017
This article is Open Access
Download Citation
RSC Adv., 2017,7, 1572-1580
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Enhancement of the antimicrobial activity of cinnamon essential oil-loaded electrospun nanofilm by the incorporation of lysozyme
K. Feng, P. Wen, H. Yang, N. Li, W. Y. Lou, M. H. Zong and H. Wu,RSC Adv., 2017, 7, 1572DOI: 10.1039/C6RA25977D
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