Theanine attenuates memory impairments induced by klotho gene depletion in mice (original) (raw)
* Corresponding authors
a Neuropsychopharmacology and Toxicology Program, College of Pharmacy, Kangwon National University, Chunchon 24341, Republic of Korea
E-mail: kimhc@kangwon.ac.kr, shinej@kangwon.ac.kr
Fax: +82 33 259 5631
Tel: +82 33 250 6917
b Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul 06974, Republic of Korea
c Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 06974, Republic of Korea
d Department of Pharmacology, School of Pharmacy, Sungkyunkwan University, Suwon 16419, Korea
e Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University, Seoul 05029, Republic of Korea
f Advanced Diagnostic System Research Laboratory, Fujita Health University Graduate School of Health Sciences, Aichi 470-1192, Japan
g Aino University, Ibaragi, Japan
h Section of Prophylactic Pharmacology, Kanazawa University Venture Business Laboratory, Kanazawa, Ishikawa 920-1192, Japan
Abstract
Theanine (γ-glutamylethylamide), an amino acid in tea, is a putative neuroprotective and antioxidant compound capable of improving lifespan and cognitive function. Because we previously reported cognitive dysfunction in klotho mutant mice via down-regulation of janus kinase 2 (JAK2) and signal transducer and activator of transcription3 (STAT3), M1 muscarinic cholinergic receptor (M1 mAChR), and ERK signaling, we, therefore, investigated whether self-administration of theanine affects memory dysfunction in response to klotho gene depletion in mice, and whether theanine modulates the JAK2/STAT3, M1 mAChR, and ERK signaling network. Theanine significantly attenuated memory impairments in klotho mutant mice. Moreover, theanine self-administration significantly attenuated inhibitions of JAK2/STAT3 phosphorylation, M1 mAChR expression, and ERK1/2 phosphorylation in the hippocampus of klotho mutant mice. Consistently, AG490, a JAK2/STAT3 inhibitor, dicyclomine, an M1 mAChR antagonist, or U0126, an ERK1/2 inhibitor, significantly counteracted theanine-induced attenuation of memory impairment induced by klotho gene depletion in mice. Our study suggests that theanine attenuates memory impairments in a genetic aging model via up-regulation of JAK2/STAT3, M1 mAChR, and ERK signaling.
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Article information
DOI
https://doi.org/10.1039/C8FO01577E
Article type
Paper
Submitted
06 Aug 2018
Accepted
02 Dec 2018
First published
14 Dec 2018
Download Citation
Food Funct., 2019,10, 325-332
Permissions
Theanine attenuates memory impairments induced by klotho gene depletion in mice
B. T. Nguyen, N. Sharma, E. Shin, J. H. Jeong, S. H. Lee, C. Jang, S. Nah, T. Nabeshima, Y. Yoneda and H. Kim,Food Funct., 2019, 10, 325DOI: 10.1039/C8FO01577E
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