Fluorogenic probes for mitochondria and lysosomes via intramolecular photoclick reaction (original) (raw)

* Corresponding authors

a Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, P. R. China
E-mail: liguorui@hnu.edu.cn, huangjing16@hnu.edu.cn

b Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, College of Chemistry and Chemical Engineering, Hunan University, P. R. China

Abstract

The tetrazole-based photoclick chemistry has attracted considerable attention in virtue of its good biocompatibility, exclusive molecular reaction, and spatiotemporally controllable properties. Using this photoclick reaction, we designed an in situ, real-time fluorescence imaging system that targeted mitochondria and lysosomes in a spatiotemporally controllable manner. Upon irradiation, the pyrazoline fluorophore was generated in situ by the intramolecular tetrazole-alkene cycloaddition reaction (“photo-click chemistry”). This strategy exhibits features such as fast response, high efficiency, strong fluorescence intensity without background and superior stability. In addition, by integrating with an organelle-specific group, it has a good application for subcellular targeting imaging. Furthermore, the photo-responsive moiety Tet facilitates the probes, Mt-Tet and Ly-Tet, for the super-resolution imaging of subcellular structures.

Graphical abstract: Fluorogenic probes for mitochondria and lysosomes via intramolecular photoclick reaction

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Article information

DOI

https://doi.org/10.1039/D0AN01982H

Article type

Paper

Submitted

07 Oct 2020

Accepted

07 Dec 2020

First published

09 Dec 2020

Download Citation

Analyst, 2021,146, 1369-1375

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