Identification and isolation of anti-pneumonia bioactive compounds from Opuntia ficus-indica fruit waste peels (original) (raw)
* Corresponding authors
a Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, Cairo, Egypt
E-mail: welkady@fue.edu.eg
b Arab Company for Pharmaceuticals and Medicinal Plants (Mepaco Medifood), Cairo, Egypt
c Regional Center for Mycology and Biotechnology (RCMB), Al-Azhar University, Cairo, Egypt
Abstract
Prickly pear fruit peel constitutes a high percentage of the fruit and could be a natural, economic agro-industrial waste of potential use in the nutraceutical industry. This study aimed to isolate and characterize the main constituents of the fruit peel and evaluate its antibacterial activity. A methanol extract was successively fractionated using hexane, chloroform and ethyl acetate. The _n_-hexane fraction was evaluated for its fatty acid content using gas chromatography mass spectrometry (GC-MS), revealing linolenic acid (omega-3) as the major fatty acid (60.56%), while an ethyl acetate fraction was analyzed using ultra-performance liquid chromatography electrospray tandem mass spectrometry (UPLC-ESI-MS/MS), resulting in the identification of 6 phenolic acids and 9 flavonoids, where caffeic acid (43.69%) and quercetin (14%) were found the most abundant. The ethyl acetate fraction was subjected to column chromatography, resulting in the isolation of four flavanols, viz. astragalin (1), quercetin 5,4′-dimethyl ether (2), isorhamnetin-3-_O_-glucoside (3) and isorhamnetin (4). Antibacterial evaluation revealed that the EtOAc fraction is the most potent active fraction against the selected pneumonia pathogens, and quercetin 5,4′-dimethyl ether (2) is the most active among the isolated compounds. Virtual docking of the isolated compounds showed promising in silico anti-quorum sensing efficacy, indicating that they could represent natural antibacterial agents. These findings indicate that the unused waste from prickly pear fruits contains valuable constituents that have beneficial potential against some pneumonia pathogens.
You have access to this article
Please wait while we load your content... Something went wrong. Try again?
Article information
DOI
https://doi.org/10.1039/D0FO00817F
Article type
Paper
Submitted
29 Mar 2020
Accepted
11 May 2020
First published
12 May 2020
Download Citation
Food Funct., 2020,11, 5275-5283
Permissions
Identification and isolation of anti-pneumonia bioactive compounds from Opuntia ficus-indica fruit waste peels
W. M. Elkady, M. M. Bishr, M. M. Abdel-Aziz and O. M. Salama,Food Funct., 2020, 11, 5275DOI: 10.1039/D0FO00817F
To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.
If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.
If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.
Read more about how to correctly acknowledge RSC content.