BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer (original) (raw)

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1 Department of Dermatology, University of California, San Francisco, San Francisco, CA 94121, USA

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Received:

20 November 2008

Revision received:

05 February 2009

Accepted:

05 February 2009

Published:

12 February 2009

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Shahana Majid, Altaf A. Dar, Ardalan E. Ahmad, Hiroshi Hirata, Kazumori Kawakami, Varahram Shahryari, Sharanjot Saini, Yuichiro Tanaka, Angela V. Dahiya, Gaurav Khatri, Rajvir Dahiya, BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer , Carcinogenesis, Volume 30, Issue 4, April 2009, Pages 662–670, https://doi.org/10.1093/carcin/bgp042
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Abstract

BTG3 / ANA / APRO4 has been reported to be a tumor suppressor gene in some malignancies. It constitutes important negative regulatory mechanism for Src-mediated signaling, a negative regulator of the cell cycle and inhibits transcription factor E2F1. We report that BTG3 is downregulated in renal cancer and that the mechanism of inactivation is through promoter hypermethylation. Quantitative real-time polymerase chain reaction (PCR) showed that BTG3 was downregulated in cancer tissues and cells. Genistein and 5-aza-2′-deoxycytidine (5Aza-C) induced BTG3 messenger RNA (mRNA) expression in A498, ACHN and HEK-293 renal cell carcinoma (RCC) cell lines. Bisulfite-modified PCR and DNA sequencing results showed complete methylation of BTG3 promoter in tumor samples and cancer cell lines. Genistein and 5Aza-C treatment significantly decreased promoter methylation, reactivating BTG3 expression. Chromatin immunoprecipitation assay revealed that genistein and 5Aza-C increased levels of acetylated histones 3, 4, 2H3K4, 3H3K4 and RNA polymerase II at the BTG3 promoter indicative of active histone modifications. Enzymatic assays showed genistein and 5Aza-C decreased DNA Methyltransferase, methyl-CpG-binding domain 2 activity and increased HAT activity. Cell cycle and 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide cell proliferation assays showed that genistein has antiproliferative effect on cancer cell growth through induction of cell cycle arrest. This is the first report to show that BTG3 is epigenetically silenced in RCC and can be reactivated by genistein-induced promoter demethylation and active histone modification. Genistein had similar effects to that of 5Aza-C, which is a potent demethylating agent with high toxicity and instability. Genistein being a natural, non-toxic, dietary isoflavone is effective in retarding the growth of RCC cells, making it a promising candidate for epigenetic therapy in renal carcinoma.

© The Author 2009. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Topic:

• polymerase chain reaction
• immunoprecipitation
• cell cycle
• cancer
• bromides
• cell lines
• chromatin
• dna
• dna modification methylases
• tumor suppressor genes
• genistein
• histones
• methylation
• tetrazolium salts
• neoplasms
• hypermethylation
• cell cycle arrest
• tumor cells, malignant
• renal cancer
• demethylation

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