Genetic Functions Promoting Homologous Recombination in Escherichia coli: A Study of Inversions in Phage Λ (original) (raw)
Journal Article
,
Institute of Molecular Biology
, University of Oregon, Eugene, Oregon 97403
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Fred Hutchinson Cancer Research Center
, 1124 Columbia Street, Seattle, Washington 98104
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Institute of Molecular Biology
, University of Oregon, Eugene, Oregon 97403, and Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington 98104
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Accepted:
13 September 1986
Published:
01 January 1987
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Don G Ennis, Susan K Amundsen, Gerald R Smith, Genetic Functions Promoting Homologous Recombination in Escherichia coli: A Study of Inversions in Phage Λ, Genetics, Volume 115, Issue 1, 1 January 1987, Pages 11–24, https://doi.org/10.1093/genetics/115.1.11
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ABSTRACT
We have studied homologous recombination in a derivative of phage λ containing two 1.4-kb repeats in inverted orientation. Inversion of the intervening 2.5-kb segment occurred efficiently by the Escherichia coli RecBC pathway but markedly less efficiently by the λ Red pathway or the E. coli RecE or RecF pathways. Inversion by the RecBCD pathway was stimulated by Chi sites located to the right of the invertible segment; this stimulation decreased exponentially by a factor of about 2 for each 2.2 kb between the invertible segment and the Chi site. In addition to RecA protein and RecBCD enzyme, inversion by the RecBC pathway required single-stranded DNA binding protein, DNA gyrase, DNA polymerase I and DNA ligase. Inversion appeared to occur either intra- or intermolecularly. These results are discussed in the framework of a current molecular model for the RecBC pathway of homologous recombination.
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© Genetics 1987
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