Intrachromosomal Recombination in Saccharomyces cerevisiae : Reciprocal Exchange in an Inverted Repeat and Associated Gene Conversion (original) (raw)

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,

Department of Biochemistry

, New York University, New York, New York 10016

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Department of Biochemistry

, New York University, New York, New York 10016

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Published:

01 December 1987

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Kerry K Willis, Hannah L Klein, Intrachromosomal Recombination in Saccharomyces cerevisiae : Reciprocal Exchange in an Inverted Repeat and Associated Gene Conversion, Genetics, Volume 117, Issue 4, 1 December 1987, Pages 633–643, https://doi.org/10.1093/genetics/117.4.633
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ABSTRACT

Intrachromosomal gene conversion has not shown a strong association with reciprocal exchanges. However, reciprocal exchanges do occur between intrachromosomal repeats. To understand the relationship between reciprocal exchange and gene conversion in repeated sequences the recombi- nation behavior of an inverted repeat was studied. We have found that in one orientation a single copy of the Kan r gene of the bacterial transposon Tn903 flanked by part of the inverted repeats IS903 does not give G418 resistance in Saccharomyces cerevisiae. A reciprocal exchange in the IS903 repeats inverts the Kan r gene, which then gives G4 18 resistance in a single copy. Using this as a selection for intrachromosomal reciprocal exchange we have introduced multiple restriction site heterologies into the IS903 repeats and examined the crossover products for associated gene conversions. Approximately 50% of crossovers, both in mitosis and meiosis, were associated with a gene conversion. This suggests that these crossovers result from an intermediate that gives a gene conversion in 50% of the events, that is, both reciprocal exchange and gene conversion between repeated sequences have a common origin. The data are most consistent with a heteroduplex mismatch repair mechanism.

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© Genetics 1987

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