Loslp, Involved in Yeast Pre-tRNA Splicing, Positively Regulates Members of the SOL Gene Family (original) (raw)
Journal Article
,
Search for other works by this author on:
,
Search for other works by this author on:
Corresponding author: Anita K. Hopper, Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, 500 University Dr., Hershey, PA 17033-0850, E-mail: ahopper@cor-mail.biochem.hmc.psu.edu
Search for other works by this author on:
Present address; Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, MA 01605.
Received:
26 December 1995
Cite
W-C Shen, D R Stanford, A K Hopper, Loslp, Involved in Yeast Pre-tRNA Splicing, Positively Regulates Members of the SOL Gene Family, Genetics, Volume 143, Issue 2, 1 June 1996, Pages 699–712, https://doi.org/10.1093/genetics/143.2.699
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
To understand the role of Los1p in pre-tRNA splicing, we sought los1 multicopy suppressors. We found SOLl that suppresses both point and null LOS1 mutations. Since, when fused to the Gal4p DNA-binding domain, Los1p activates transcription, we tested whether Los1p regulates SOL1. We found that los1 mutants have depleted levels of SOL1 mRNA and Sollp. Thus, LOS1 appears to positively regulate SOL1. SOL1 belongs to a multigene family with at least two additional members, SOL2 and SOL3. Sol proteins have extensive similarity to an unusual group of glucose-6phosphate dehydrogenases. As the similarities are restricted to areas separate from the catalytic domain, these G6PDs may have more than one function. The SOL family appears to be unessential since cells with a triple disruption of all three SOL genes are viable. SOL gene disruptions negatively affect tRNA-mediated nonsense suppression and the severity increases with the number of mutant SOL genes. However, tRNA levels do not vary with either multicopy SOL genes or with SOL disruptions. Therefore, the Sol proteins affect tRNA expression/function at steps other than transcription or splicing. We propose that LOS1 regulates gene products involved in tRNA expression/function as well as pre-tRNA splicing.
Communicating editor: F. Winston
This content is only available as a PDF.
Author notes
1
Present address; Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, MA 01605.
© Genetics 1996
Citations
Views
Altmetric
Metrics
Total Views 83
0 Pageviews
83 PDF Downloads
Since 1/1/2021
Month: | Total Views: |
---|---|
January 2021 | 4 |
March 2021 | 1 |
May 2021 | 2 |
July 2021 | 1 |
August 2021 | 1 |
October 2021 | 5 |
November 2021 | 8 |
December 2021 | 4 |
January 2022 | 1 |
February 2022 | 1 |
March 2022 | 1 |
April 2022 | 5 |
May 2022 | 1 |
June 2022 | 1 |
July 2022 | 3 |
August 2022 | 1 |
October 2022 | 1 |
November 2022 | 1 |
December 2022 | 2 |
January 2023 | 3 |
February 2023 | 1 |
April 2023 | 1 |
June 2023 | 2 |
August 2023 | 2 |
October 2023 | 1 |
December 2023 | 4 |
January 2024 | 4 |
February 2024 | 3 |
March 2024 | 3 |
April 2024 | 2 |
May 2024 | 1 |
July 2024 | 1 |
August 2024 | 8 |
September 2024 | 2 |
October 2024 | 1 |
×
Email alerts
Citing articles via
More from Oxford Academic