TEMPERATURE-SENSITIVE MUTANTS FOR THE REPLICATION OF PLASMIDS IN ESCHERICHIA COLI I. ISOLATION AND SPECIFICITY OF HOST AND PLASMID MUTATIONS (original) (raw)
Journal Article
,
Department of Biology
, University of California, San Diego, La Jolla, California 92037
Search for other works by this author on:
Department of Biology
, University of California, San Diego, La Jolla, California 92037
Search for other works by this author on:
Received:
06 November 1972
Cite
David T Kingsbury, Donald R Helinski, TEMPERATURE-SENSITIVE MUTANTS FOR THE REPLICATION OF PLASMIDS IN ESCHERICHIA COLI I. ISOLATION AND SPECIFICITY OF HOST AND PLASMID MUTATIONS, Genetics, Volume 74, Issue 1, 1 May 1973, Pages 17–31, https://doi.org/10.1093/genetics/74.1.17
Close
Navbar Search Filter Mobile Enter search term Search
ABSTRACT
Temperature-sensitive mutants of Escherichia coli defective in the replication of the plasmid colicinogenic factor E1 (ColE1) were isolated following mutagenesis of E. coli K12 strain carrying the ColE1 factor. Following the mutagenic treatment an enrichment procedure utilizing the replacement of thymine with bromouracil in the ColE1 DNA duplicated at the restrictive temperature was used. The mutants isolated following this enrichment step were the result of a mutation event either in the host chromosome or in the ColE1 plasmid. The host mutants fell into three phenotypic classes based on the effect each mutation had on the maintenance of a variety of other extrachromosomal DNA elements. Phenotypic class I mutations affected all E. coli plasmids, both the I and F sex factor types as well as the ColE1 factor. Phenotypic class II mutations affected the maintenance of the ColE1 and the F sex factor type plasmids and not the I type, while phenotypic class III mutations affected only ColE1 replication. None of these mutations was found to have a significant effect on the replication of the E. coli chromosome. The plasmid-linked mutations fell into two phenotypic classes on the basis of the ability of the Flac episome to complement the mutation in the ColE1 plasmid.
This content is only available as a PDF.
© Genetics 1973
Citations
Views
Altmetric
Metrics
Total Views 74
0 Pageviews
74 PDF Downloads
Since 1/1/2021
Month: | Total Views: |
---|---|
January 2021 | 3 |
March 2021 | 1 |
July 2021 | 2 |
August 2021 | 1 |
October 2021 | 2 |
November 2021 | 3 |
January 2022 | 1 |
February 2022 | 1 |
March 2022 | 2 |
April 2022 | 6 |
June 2022 | 2 |
July 2022 | 1 |
August 2022 | 1 |
October 2022 | 2 |
November 2022 | 1 |
December 2022 | 1 |
February 2023 | 1 |
April 2023 | 1 |
June 2023 | 6 |
July 2023 | 2 |
August 2023 | 1 |
September 2023 | 2 |
October 2023 | 1 |
December 2023 | 3 |
February 2024 | 3 |
March 2024 | 2 |
April 2024 | 3 |
May 2024 | 2 |
July 2024 | 4 |
August 2024 | 10 |
September 2024 | 3 |
×
Email alerts
Citing articles via
More from Oxford Academic