Heterogeneity in Telomere Length of Human Chromosomes (original) (raw)

Journal Article

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1

Terry Fox Laboratory, B.C. Cancer Agency

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601 West 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada

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Department of Medicine, University of British Columbia

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Vancouver, B.C., Canada

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Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden

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Wassenaarseweg 72, 2333 Al Leiden, The Netherlands

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Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden

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Wassenaarseweg 72, 2333 Al Leiden, The Netherlands

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Terry Fox Laboratory, B.C. Cancer Agency

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601 West 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada

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1

Terry Fox Laboratory, B.C. Cancer Agency

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601 West 10th Avenue, Vancouver, B.C., V5Z 1L3, Canada

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Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden

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Wassenaarseweg 72, 2333 Al Leiden, The Netherlands

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Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden

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Wassenaarseweg 72, 2333 Al Leiden, The Netherlands

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Sylvius Laboratory, Department of Cytochemistry and Cytometry, University of Leiden

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Wassenaarseweg 72, 2333 Al Leiden, The Netherlands

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Received:

15 January 1996

Accepted:

21 February 1996

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Peter M. Lansdorp, Nico P. Verwoerd, Frans M. van de Rijke, Visia Dragowska, Marie-Térèse Little, Roeland W. Dirks, Anton K. Raap, Hans J. Tanke, Heterogeneity in Telomere Length of Human Chromosomes, Human Molecular Genetics, Volume 5, Issue 5, May 1996, Pages 685–691, https://doi.org/10.1093/hmg/5.5.685
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Abstract

Vertebrate chromosomes terminate in variable numbers of T 2 AG 3 nucleotide repeats. In order to study telomere repeats at individual chromosomes, we developed novel, quantitative fluorescence in situ hybridization procedures using labeled (C 3 TA 2 ) 3 peptide nucleic acid and digital imaging microscopy. Telomere fluorescence intensity values from metaphase chromosomes of cultured human hematopoietic cells decreased with the replication history of the cells, varied up to six-fold within a metaphase, and were similar between sister chromatid telomeres. Surprisingly, telomere fluorescence intensity values within normal adult bone marrow metaphases did not show a normal distribution, suggesting that a minimum number of repeats at each telomere is required and/or maintained during normal hematopoiesis.

© 1996 Oxford University Press

Topic:

• fluorescent in situ hybridization
• heterogeneity
• adult
• chromatids
• chromosomes
• chromosomes, human
• fluorescence
• hematopoiesis
• mitotic metaphase
• peptide nucleic acids
• telomere
• vertebrates
• bone marrow

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