A DNA Probe to Identify Enterohemorrhagic Escherichia coli of 0157:H7 and Other Serotypes That Cause Hemorrhagic Colitis and Hemolytic Uremic Syndrome (original) (raw)
Journal Article
,
From the
Center for Vaccine Development, Division of Geographic Medicine, Department of Medicine, and the Division of Infectious Diseases and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine
,
Baltimore, Maryland
; the
Laboratory Center for Disease Control
,
Ottawa, Canada
; the
Department of Microbiology, University of Chile Faculty of Medicine
,
Area Oriente, Santiago, Chile
; the
Institut für Medizinische Mikrobiologie und Immunologie, Universitats-Krankenhaus Eppendorf
,
Hamburg, Federal Republic of Germany
; and the
Centers for Disease Control
,
Atlanta, Georgia
Please address requests for reprints to Dr. M. M. Levine, Center for Vaccine Development, University of Maryland School of Medicine, 10 South Pine Street, Baltimore, Maryland 21201.
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,
From the
Center for Vaccine Development, Division of Geographic Medicine, Department of Medicine, and the Division of Infectious Diseases and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine
,
Baltimore, Maryland
; the
Laboratory Center for Disease Control
,
Ottawa, Canada
; the
Department of Microbiology, University of Chile Faculty of Medicine
,
Area Oriente, Santiago, Chile
; the
Institut für Medizinische Mikrobiologie und Immunologie, Universitats-Krankenhaus Eppendorf
,
Hamburg, Federal Republic of Germany
; and the
Centers for Disease Control
,
Atlanta, Georgia
Search for other works by this author on:
,
From the
Center for Vaccine Development, Division of Geographic Medicine, Department of Medicine, and the Division of Infectious Diseases and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine
,
Baltimore, Maryland
; the
Laboratory Center for Disease Control
,
Ottawa, Canada
; the
Department of Microbiology, University of Chile Faculty of Medicine
,
Area Oriente, Santiago, Chile
; the
Institut für Medizinische Mikrobiologie und Immunologie, Universitats-Krankenhaus Eppendorf
,
Hamburg, Federal Republic of Germany
; and the
Centers for Disease Control
,
Atlanta, Georgia
Search for other works by this author on:
,
From the
Center for Vaccine Development, Division of Geographic Medicine, Department of Medicine, and the Division of Infectious Diseases and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine
,
Baltimore, Maryland
; the
Laboratory Center for Disease Control
,
Ottawa, Canada
; the
Department of Microbiology, University of Chile Faculty of Medicine
,
Area Oriente, Santiago, Chile
; the
Institut für Medizinische Mikrobiologie und Immunologie, Universitats-Krankenhaus Eppendorf
,
Hamburg, Federal Republic of Germany
; and the
Centers for Disease Control
,
Atlanta, Georgia
Search for other works by this author on:
,
From the
Center for Vaccine Development, Division of Geographic Medicine, Department of Medicine, and the Division of Infectious Diseases and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine
,
Baltimore, Maryland
; the
Laboratory Center for Disease Control
,
Ottawa, Canada
; the
Department of Microbiology, University of Chile Faculty of Medicine
,
Area Oriente, Santiago, Chile
; the
Institut für Medizinische Mikrobiologie und Immunologie, Universitats-Krankenhaus Eppendorf
,
Hamburg, Federal Republic of Germany
; and the
Centers for Disease Control
,
Atlanta, Georgia
Search for other works by this author on:
,
From the
Center for Vaccine Development, Division of Geographic Medicine, Department of Medicine, and the Division of Infectious Diseases and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine
,
Baltimore, Maryland
; the
Laboratory Center for Disease Control
,
Ottawa, Canada
; the
Department of Microbiology, University of Chile Faculty of Medicine
,
Area Oriente, Santiago, Chile
; the
Institut für Medizinische Mikrobiologie und Immunologie, Universitats-Krankenhaus Eppendorf
,
Hamburg, Federal Republic of Germany
; and the
Centers for Disease Control
,
Atlanta, Georgia
Search for other works by this author on:
,
From the
Center for Vaccine Development, Division of Geographic Medicine, Department of Medicine, and the Division of Infectious Diseases and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine
,
Baltimore, Maryland
; the
Laboratory Center for Disease Control
,
Ottawa, Canada
; the
Department of Microbiology, University of Chile Faculty of Medicine
,
Area Oriente, Santiago, Chile
; the
Institut für Medizinische Mikrobiologie und Immunologie, Universitats-Krankenhaus Eppendorf
,
Hamburg, Federal Republic of Germany
; and the
Centers for Disease Control
,
Atlanta, Georgia
Search for other works by this author on:
,
From the
Center for Vaccine Development, Division of Geographic Medicine, Department of Medicine, and the Division of Infectious Diseases and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine
,
Baltimore, Maryland
; the
Laboratory Center for Disease Control
,
Ottawa, Canada
; the
Department of Microbiology, University of Chile Faculty of Medicine
,
Area Oriente, Santiago, Chile
; the
Institut für Medizinische Mikrobiologie und Immunologie, Universitats-Krankenhaus Eppendorf
,
Hamburg, Federal Republic of Germany
; and the
Centers for Disease Control
,
Atlanta, Georgia
Search for other works by this author on:
From the
Center for Vaccine Development, Division of Geographic Medicine, Department of Medicine, and the Division of Infectious Diseases and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine
,
Baltimore, Maryland
; the
Laboratory Center for Disease Control
,
Ottawa, Canada
; the
Department of Microbiology, University of Chile Faculty of Medicine
,
Area Oriente, Santiago, Chile
; the
Institut für Medizinische Mikrobiologie und Immunologie, Universitats-Krankenhaus Eppendorf
,
Hamburg, Federal Republic of Germany
; and the
Centers for Disease Control
,
Atlanta, Georgia
Search for other works by this author on:
Received:
18 November 1986
Cite
Myron M. Levine, Jian-guo Xu, James B. Kaper, Hermy Lior, Valeria Prado, Ben Tall, James Nataro, Helge Karch, Kaye Wachsmuth, A DNA Probe to Identify Enterohemorrhagic Escherichia coli of 0157:H7 and Other Serotypes That Cause Hemorrhagic Colitis and Hemolytic Uremic Syndrome, The Journal of Infectious Diseases, Volume 156, Issue 1, July 1987, Pages 175–182, https://doi.org/10.1093/infdis/156.1.175
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Abstract
Enterohemorrhagic Escherichia coli (EHEC) cause hemorrhagic colitis and hemolytic uremic syndrome (HUS), make potent cytotoxins (Verotoxins [VT] or Shiga-like toxins), and possess a plasmid (∼60 megadaltons) that encodes a new fimbrial antigen and promotes attachment to epithelial cells.Weevaluated the use of a DNA probe, prepared from a 3.4-kilobase segment of the EHEC plasmid, to detect EHEC. The probe hybridized with 106 (99%) of 107 O157:H7 and 34 (77%) of 44 O26:H11, VT-positive strains from patients with colitis, HUS, and diarrheal disease and hybridized with 21 (81%) of 26 VT-positive E. coli of serotypes other than O157:H7 or O26:H11 from patients with hemorrhagic colitis and HUS. Weexamined 601 other strains, including 18 serotype O26 isolates of H types other than H11, 306 enteropathogenic E. coli, 60 enteroinvasive E. coli, 119 enterotoxigenic E. coli, and 20 isolates from the urinary tract and 77 isolates from the normal intestinal flora; only one (O127:H-) was positive (specificity, 99.8%). Serotype O26:H11, previously considered a classic enteropathogenic E. coli serotype, is now shown to be EHEC.
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© 1987 by The University of Chicago
Topic:
- plasmids
- cytotoxin
- diarrhea
- hemolytic-uremic syndrome
- antigens
- colitis
- dna probes
- shiga toxins
- urinary tract
- epithelial cells
- intestinal bacteria
- hemorrhagic colitis
- enterohemorrhagic escherichia coli
- serotype
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