Steroid Hormone Receptor Status of Mouse Mammary Stem Cells (original) (raw)

Journal Article

,

Marie-Liesse Asselin-Labat

Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)

Correspondence to: Jane E. Visvader, PhD, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia (e-mail: visvader@wehi.edu.au ) or Geoffrey J. Lindeman, FRACP, PhD, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia (e-mail: lindeman@wehi.edu.au ).

Search for other works by this author on:

,

Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)

Search for other works by this author on:

,

Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)

Search for other works by this author on:

,

Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)

Search for other works by this author on:

,

Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)

Search for other works by this author on:

,

Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)

Search for other works by this author on:

,

Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)

Search for other works by this author on:

Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)

Search for other works by this author on:

Revision received:

02 May 2006

• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

Marie-Liesse Asselin-Labat, Mark Shackleton, John Stingl, François Vaillant, Natasha C. Forrest, Connie J. Eaves, Jane E. Visvader, Geoffrey J. Lindeman, Steroid Hormone Receptor Status of Mouse Mammary Stem Cells, JNCI: Journal of the National Cancer Institute, Volume 98, Issue 14, 19 July 2006, Pages 1011–1014, https://doi.org/10.1093/jnci/djj267
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

The estrogen receptor α (ERα), progesterone receptor (PR), and erbB2 (Her2 in humans) are important prognostic markers of human breast cancer, and they are variably expressed in different subtypes of breast cancer. The basal subtype, for example, is negative for ERα, PR, and Her2 by immunohistochemistry. We investigated the expression of these signaling molecules in enriched populations of mouse mammary stem cells and luminal cells that were isolated according to their differential expression of CD24 and the α6β1-integrin complex. We found that the basal population, which is enriched in mouse mammary stem cells, did not express ERα, PR, or ErbB2/Her2 but did express epidermal growth factor receptor (EGFR)/ErbB1, whereas the subset of cells enriched for luminal cells expressed ERα (37% of cells) and PR (40% of cells) but not ErbB2/Her2 or EGFR/ErbB1. Ovariectomy confirmed the importance of estrogen signaling to luminal cell proliferation but had no effect on the size of the mouse mammary stem cell-enriched population. Thus, mouse mammary stem cells were negative for ERα, PR, and ErbB2 and appeared to share common properties with poor-prognosis basal breast cancer.

© The Author 2006. Published by Oxford University Press.

Topic:

• signal transduction
• immunohistochemistry
• cell proliferation
• integrins
• estrogen
• stem cells
• genes, erbb-2
• ovariectomy
• epidermal growth factor receptors
• receptor, erbb-2
• estrogen receptors
• receptors, progesterone
• mice
• phenobarbital
• breast cancer
• prognostic marker
• steroid hormone receptor
• molecule

You do not currently have access to this article.

Personal account

• Sign in with email/username & password
• Get email alerts
• Save searches
• Purchase content
• Activate your purchase/trial code
• Add your ORCID iD

Get help with access

Institutional access

Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:

IP based access

Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.

Sign in through your institution

Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.

1. Click Sign in through your institution.
2. Select your institution from the list provided, which will take you to your institution's website to sign in.
3. When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
4. Following successful sign in, you will be returned to Oxford Academic.

If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.

Sign in with a library card

Enter your library card number to sign in. If you cannot sign in, please contact your librarian.

Society Members

Society member access to a journal is achieved in one of the following ways:

Sign in through society site

Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:

1. Click Sign in through society site.
2. When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
3. Following successful sign in, you will be returned to Oxford Academic.

If you do not have a society account or have forgotten your username or password, please contact your society.

Sign in using a personal account

Some societies use Oxford Academic personal accounts to provide access to their members. See below.

Personal account

A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.

Some societies use Oxford Academic personal accounts to provide access to their members.

Viewing your signed in accounts

Click the account icon in the top right to:

• View your signed in personal account and access account management features.
• View the institutional accounts that are providing access.

Signed in but can't access content

Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.

Institutional account management

For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.

Purchase

Short-term Access

To purchase short-term access, please sign in to your personal account above.

Don't already have a personal account? Register

Steroid Hormone Receptor Status of Mouse Mammary Stem Cells - 24 Hours access

EUR €38.00

GBP £33.00

USD $41.00

Rental

This article is also available for rental through DeepDyve.

Citations

Views

Altmetric

Metrics

Total Views 1,806

1,278 Pageviews

528 PDF Downloads

Since 1/1/2017

Citations

232 Web of Science

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic