Steroid Hormone Receptor Status of Mouse Mammary Stem Cells (original) (raw)
Journal Article
Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)
Correspondence to: Jane E. Visvader, PhD, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia (e-mail: [email protected] ) or Geoffrey J. Lindeman, FRACP, PhD, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia (e-mail: [email protected] ).
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Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)
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Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)
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Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)
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Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)
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Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)
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Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)
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Affiliations of authors: VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia (MLAL, MS, FV, NCF, JEV, GJL); University of Melbourne, Victoria, Australia (MS, GJL); Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada (JS, CJE); StemCell Technologies Inc., Vancouver, British Columbia, Canada (JS); Department of Clinical Haematology and Medical Oncology, Royal Melbourne Hospital, Melbourne, Victoria, Australia (GJL)
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Revision received:
02 May 2006
Cite
Marie-Liesse Asselin-Labat, Mark Shackleton, John Stingl, François Vaillant, Natasha C. Forrest, Connie J. Eaves, Jane E. Visvader, Geoffrey J. Lindeman, Steroid Hormone Receptor Status of Mouse Mammary Stem Cells, JNCI: Journal of the National Cancer Institute, Volume 98, Issue 14, 19 July 2006, Pages 1011–1014, https://doi.org/10.1093/jnci/djj267
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Abstract
The estrogen receptor α (ERα), progesterone receptor (PR), and erbB2 (Her2 in humans) are important prognostic markers of human breast cancer, and they are variably expressed in different subtypes of breast cancer. The basal subtype, for example, is negative for ERα, PR, and Her2 by immunohistochemistry. We investigated the expression of these signaling molecules in enriched populations of mouse mammary stem cells and luminal cells that were isolated according to their differential expression of CD24 and the α6β1-integrin complex. We found that the basal population, which is enriched in mouse mammary stem cells, did not express ERα, PR, or ErbB2/Her2 but did express epidermal growth factor receptor (EGFR)/ErbB1, whereas the subset of cells enriched for luminal cells expressed ERα (37% of cells) and PR (40% of cells) but not ErbB2/Her2 or EGFR/ErbB1. Ovariectomy confirmed the importance of estrogen signaling to luminal cell proliferation but had no effect on the size of the mouse mammary stem cell-enriched population. Thus, mouse mammary stem cells were negative for ERα, PR, and ErbB2 and appeared to share common properties with poor-prognosis basal breast cancer.
© The Author 2006. Published by Oxford University Press.
Topic:
- signal transduction
- immunohistochemistry
- cell proliferation
- integrins
- estrogen
- stem cells
- genes, erbb-2
- ovariectomy
- epidermal growth factor receptors
- receptor, erbb-2
- estrogen receptors
- receptors, progesterone
- mice
- phenobarbital
- breast cancer
- prognostic marker
- steroid hormone receptor
- molecule
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