Alignment of nucleosomes along DNA and organization of spacer DNA in Drosophila chromatin (original) (raw)
Journal Article
,
Institute of Molecular Biology, Academy of Sciences of the USSR
Moscor, USSR
Search for other works by this author on:
,
Institute of Molecular Biology, Academy of Sciences of the USSR
Moscor, USSR
Search for other works by this author on:
,
Institute of Molecular Biology, Academy of Sciences of the USSR
Moscor, USSR
Search for other works by this author on:
,
Institute of Molecular Biology, Academy of Sciences of the USSR
Moscor, USSR
Search for other works by this author on:
Institute of Molecular Biology, Academy of Sciences of the USSR
Moscor, USSR
Search for other works by this author on:
Revision received:
14 June 1982
PDF Cite
V.L. Karpov, S.G. Bavykin, O.V. Preobrazhenskaya, A.V. Belyavsky, A.D. Mirzabekov, Alignment of nucleosomes along DNA and organization of spacer DNA in Drosophila chromatin , Nucleic Acids Research, Volume 10, Issue 14, 24 July 1982, Pages 4321–4337, https://doi.org/10.1093/nar/10.14.4321
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
A series of mono- and dinucleosomal DNAs characterized by an about ten-base periodicity in the size were revealed in the micrococcal nuclease digests of Drosophila chromatin which have 180-5 base pair (bp) nucleosomal repeat. 20, 30, and 40 bp spacers were found to be predominant in chromatin by trimming DNA in dinucleosmes to the core positian. Among several identified mononucleosmes (MN), MN 170 , MN 180 and MN 190 were isolated from different sources (the figures indicate the DNA length in bp). The presence of the 10, 20, and 30 bp long spacers was shown in these mononucleosomes by experiments. The interaction of histone H3 with the spacer in the Drosohila MN 180 particle was also shown by the crosslinking /5/. We conclude from them results that the 10 n bp long intercore DNA (n=2 , 3 and 4) is organized by histone H3, in particular, and together with the core DNA forms a continuous superhelix. Taken together, these data suggest that Drosphila chromatin consists of the regularly aligned and tightly packed MN 180 , as a repeating unit, containing 10 and 20 bp spacers at the two ends of 180 bp DNA. Within the asymmetric and randomly oriented in chromatin MN 180 , the cores occupy two alternative positions spaced by 10 bp.
This content is only available as a PDF.
© IRL Press Limited
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.
Citations
Views
Altmetric
Metrics
Total Views 48
9 Pageviews
39 PDF Downloads
Since 1/1/2017
| Month: | Total Views: |
|---|---|
| January 2017 | 3 |
| February 2017 | 1 |
| March 2017 | 1 |
| October 2017 | 1 |
| November 2017 | 1 |
| December 2017 | 7 |
| January 2018 | 2 |
| February 2018 | 5 |
| March 2018 | 7 |
| April 2018 | 6 |
| July 2018 | 1 |
| April 2021 | 1 |
| April 2022 | 1 |
| August 2022 | 1 |
| December 2023 | 1 |
| April 2024 | 4 |
| May 2024 | 1 |
| June 2024 | 1 |
| July 2024 | 2 |
| August 2024 | 1 |
Citations
39 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic