Characterization of a halobacterial gene affecting bacterio-opsin gene expression (original) (raw)

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Department of Biochemistry and Biophysics, University of California

San Francisco, CA 94143, USA

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Department of Biochemistry and Biophysics, University of California

San Francisco, CA 94143, USA

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,

Department of Biochemistry and Biophysics, University of California

San Francisco, CA 94143, USA

Search for other works by this author on:

Department of Biochemistry and Biophysics, University of California

San Francisco, CA 94143, USA

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Revision received:

24 September 1984

Accepted:

24 September 1984

Published:

25 October 1984

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Mary Betlach, James Friedman, Herbert W. Boyer, Felicitas Pfeifer, Characterization of a halobacterial gene affecting bacterio-opsin gene expression, Nucleic Acids Research, Volume 12, Issue 20, 25 October 1984, Pages 7949–7959, https://doi.org/10.1093/nar/12.20.7949
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Abstract

A substantial number of spontaneous bacterio-opsin mutants of Halobacterium halobium are the result of insertion elements up to 1400 bp upstream of the bacterio-opsin ( bop ) gene. The nucleotide sequence of 1800 bp upstream of the bop gene has been determined. There is a 1118 bp open reading frame (ORE) located within this region which is transcribed and which coincides with the distribution of insertion elements upstream of the bop gene in Bop mutants. Therefore, we propose that there is a gene ( brp gene) 526 bp upstream of the bop gene. This putative gene is transcribed in the opposite direction as the . bop gene and could encode a protein of 37,500 D (359 amino acids) with a codon usage similar to bacterio-opsin. The 5' terminus of the transcript has been determined. The transcript and the bop mRNA are complementary for 13 residues near their 5 termini and both transcripts start at or near the initiating codon of the gene. Both tran scripts could form similar hairpin loop structures at their 5' termini which contain possible ribosomal binding sites. The DNA sequences immediately upstream of the bop and the brp genes have significant homologies and there is a short complementary sequence. The role of the brp gene in bacterio-opsin gene expression is unclear.

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