Non-Alu family interspersed repeats in human DNA and their transcriptional activity (original) (raw)
Journal Article
Lily Sun ,
Search for other works by this author on:
1
Biochemistry and Biophysics, University of California
Davis, CA 95616
Search for other works by this author on:
* To whom correspondence should be communicated
Search for other works by this author on:
2
Department of Microbiology and Immunology, Albert Einstein College of Medicine
Bronx, NY 10461, USA
Search for other works by this author on:
2
Department of Microbiology and Immunology, Albert Einstein College of Medicine
Bronx, NY 10461, USA
Search for other works by this author on:
Received:
18 January 1984
Accepted:
29 February 1984
PDF Cite
Lily Sun, K.Eric Paulson, Carl W. Schmid, Lisa Kadyk, Leslie Leinwand, Non-Alu family interspersed repeats in human DNA and their transcriptional activity, Nucleic Acids Research, Volume 12, Issue 6, 26 March 1984, Pages 2669–2690, https://doi.org/10.1093/nar/12.6.2669
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
Randomly selected human genomic clones have been surveyed for the presence of non-Alu family interspersed repeats. Four such families of repeats have been isolated and characterized with respect to repetition frequency, interspersion, base sequence, sequence divergence, in vitro RNA polymerase III transcription, elongation of transcripts in isolated nuclei, and in vivo transcription. The two most abundant of the four families of repeats correspond to previously reported families of repeats, namely the Kpn I family and poly (CA). We conclude that most of the highly repetitive (> 50,000 copies) human interspersed repeats have already been identified. Two lower abundance repeats families are also described here
The abundance with which each of these families is represented in nuclear RNA qualitatively corresponds to their genomic reiteration frequencies. Further, the complementary strands of each repeat family are approximately symmetrically transcribed. The abundance of these repeats in cytoplasmic RNA is qualitatively less than in nuclear RNA. The bulk of the in vivo transcriptional activity of these repeats thus appears to be nonspecific read through from other promoters
This content is only available as a PDF.
© 1984 IRL Press Ltd.
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.