Interrelations between the maturation of a 100 kDa nucleolar protein and pre rRNA synthesis in CHO cells (original) (raw)
Journal Article
,
Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS
118, route de Narbonne, 31062 Toulouse Cedex, France
Search for other works by this author on:
,
Michèle Caizergues-Ferrer
Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS
118, route de Narbonne, 31062 Toulouse Cedex, France
Search for other works by this author on:
,
Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS
118, route de Narbonne, 31062 Toulouse Cedex, France
Search for other works by this author on:
Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS
118, route de Narbonne, 31062 Toulouse Cedex, France
*To whom correspondance should be addressed
Search for other works by this author on:
Received:
13 February 1984
PDF Cite
Gérard Bouche, Michèle Caizergues-Ferrer, Béatrix Bugler, François Amalric, Interrelations between the maturation of a 100 kDa nucleolar protein and pre rRNA synthesis in CHO cells, Nucleic Acids Research, Volume 12, Issue 7, 11 April 1984, Pages 3025–3035, https://doi.org/10.1093/nar/12.7.3025
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
The synthesis of preribosomal RNA is inhibited “in vivo” and “in vitro” by the protease inhibitor leupeptin. “In vivo” leupeptin decreases by 74 % the incorporation of labeled uridine into 45S pre rRNA while the synthesis of other RNA species is only slightly decreased. “In vitro”, the elongation of already initiated pre rRNA chains that is achieved by incubation of isolated nucleoli is blocked by leupeptin. On the other hand, “in vitro” leupeptin has no direct effect on RNA polymerase I, tested in a nonspecific transcriptional system with Calf thymus DNA as template and in run off experiments with a cloned DNA containing the initiation site of the rDNA gene. A 100 kDa nucleolar protein which has been shown to be endoproteolytic cleaved “in vivo” (1) acts as an inhibitor of rDNA transcription in presence of leupeptin but produces little effect on the nonspecific transcription. In absence of the drug, the 100 kDa protein is processed in specific peptides which appeared to be similar to the “in vivo” maturation products. The possible role of the 100 kDa maturation process in the regulation of rDNA transcription is discussed.
This content is only available as a PDF.
© 1984 IRL Press Ltd.
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.
Citations
Views
Altmetric
Metrics
Total Views 74
19 Pageviews
55 PDF Downloads
Since 1/1/2017
| Month: | Total Views: |
|---|---|
| January 2017 | 3 |
| March 2017 | 1 |
| June 2017 | 3 |
| August 2017 | 1 |
| October 2017 | 1 |
| November 2017 | 1 |
| December 2017 | 5 |
| January 2018 | 5 |
| February 2018 | 3 |
| March 2018 | 7 |
| April 2018 | 8 |
| July 2019 | 1 |
| December 2020 | 1 |
| June 2021 | 1 |
| August 2021 | 1 |
| March 2022 | 1 |
| August 2022 | 1 |
| October 2022 | 2 |
| November 2022 | 1 |
| January 2023 | 1 |
| March 2023 | 1 |
| August 2023 | 1 |
| September 2023 | 1 |
| December 2023 | 1 |
| January 2024 | 1 |
| March 2024 | 1 |
| April 2024 | 3 |
| May 2024 | 4 |
| June 2024 | 3 |
| July 2024 | 3 |
| August 2024 | 6 |
| September 2024 | 1 |
Citations
130 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic