Use of complementary DNA oligomers to probe trp leader transcript secondary structures involved in transcription pausing and termination (original) (raw)
Journal Article
,
Department of Biological Sciences, Stanford University
Stanford, CA 94305, USA
Search for other works by this author on:
Department of Biological Sciences, Stanford University
Stanford, CA 94305, USA
Search for other works by this author on:
Received:
17 January 1983
Revision received:
28 February 1984
Cite
Robert Fisher, Charles Yanofsky, Use of complementary DNA oligomers to probe trp leader transcript secondary structures involved in transcription pausing and termination, Nucleic Acids Research, Volume 12, Issue 7, 11 April 1984, Pages 3295–3302, https://doi.org/10.1093/nar/12.7.3295
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
DNA oligoners were synthesized that are perfectly complementary to different segments of the tryptophan (trp) operon leader transcript. These 15 nucleotide long oligomers were used as probes of the involvement of transcript secondary structures in two processes: transcription pausing at the pause site located near base pair 90 in the leader region, and transcription termination at the attenuator. The 15-mers were complementary to the four segments of the trp leader transcript which have been shown to form the alternative seconda structures that are believed to be responsible for pausing, termination, and antitermination. Oligomers complementary to RNA segments 1 and 3 relieved termination while the 15-mer complementary to RNA segment 1 relieved pausing. 15-mers complementary to segment 2 had no effect on pausing and the oligomer complementary to segment 4 had virtually no effect on termination.
This content is only available as a PDF.
© 1984 IRL Press Ltd.
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.
Citations
Views
Altmetric
Metrics
Total Views 38
9 Pageviews
29 PDF Downloads
Since 4/1/2017
Month: | Total Views: |
---|---|
April 2017 | 2 |
August 2017 | 1 |
September 2017 | 1 |
October 2017 | 1 |
December 2017 | 5 |
January 2018 | 3 |
February 2018 | 3 |
March 2018 | 4 |
April 2018 | 3 |
July 2018 | 1 |
August 2018 | 2 |
September 2021 | 1 |
August 2022 | 1 |
October 2022 | 1 |
September 2023 | 1 |
April 2024 | 2 |
May 2024 | 1 |
June 2024 | 1 |
July 2024 | 1 |
August 2024 | 1 |
September 2024 | 2 |
Citations
13 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic