Nudeotide sequence of the chicken 5-aminolevulinate synthase gene (original) (raw)

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Adelaide University Centre for Gene Technology, Commonwealth Special Research Centre, Department of Biochemistry, University of Adelaide

South Australia

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Adelaide University Centre for Gene Technology, Commonwealth Special Research Centre, Department of Biochemistry, University of Adelaide

South Australia

Search for other works by this author on:

,

Adelaide University Centre for Gene Technology, Commonwealth Special Research Centre, Department of Biochemistry, University of Adelaide

South Australia

Search for other works by this author on:

,

Adelaide University Centre for Gene Technology, Commonwealth Special Research Centre, Department of Biochemistry, University of Adelaide

South Australia

Search for other works by this author on:

,

Adelaide University Centre for Gene Technology, Commonwealth Special Research Centre, Department of Biochemistry, University of Adelaide

South Australia

Search for other works by this author on:

,

Adelaide University Centre for Gene Technology, Commonwealth Special Research Centre, Department of Biochemistry, University of Adelaide

South Australia

Search for other works by this author on:

Adelaide University Centre for Gene Technology, Commonwealth Special Research Centre, Department of Biochemistry, University of Adelaide

South Australia

Search for other works by this author on:

Received:

10 December 1985

Accepted:

17 January 1986

Published:

11 February 1986

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Deborah J. Maguire, Adrienne R. Day, Iain A. Borthwick, Gopesh Srivastava, Peter L. Wigley, Brian K. May, William H. Elliott, Nudeotide sequence of the chicken 5-aminolevulinate synthase gene, Nucleic Acids Research, Volume 14, Issue 3, 11 February 1986, Pages 1379–1391, https://doi.org/10.1093/nar/14.3.1379
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Abstract

5-Aminolevul inate synthase, the first and rate-controlling enzyme of heme biosynthesis, is regulated in the liver by the end-product heme. To study this negative control mechanism, we have isolated the chicken gene for ALA-synthase and determined the nucleotide sequence. The structural gene is 6.9 kb long and contains 10 exons. The transcriptional start site for ALA-synthase was determined by primer extension analysis. A fragment of 291 b p from the 5′ flanking region including 34 b p of the first exon shows promoter activity when introduced upstream of a chicken hi stone H2B gene and injected into the nuclei of Xenopus laevis oocytes.

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