Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fix ABC, nif A, nif B and a novel open reading frame (original) (raw)
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Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld
4800 Bielefeld 1, FRG
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Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld
4800 Bielefeld 1, FRG
1 Present addresses: Department of Dairy and Food Microbiology, University College, Cork, Ireland
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Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld
4800 Bielefeld 1, FRG
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Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld
4800 Bielefeld 1, FRG
2 Present addresses: National Institute for Higher Education, Dublin, Ireland
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Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld
4800 Bielefeld 1, FRG
* To whom reprint requests should be sent
Search for other works by this author on:
Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld
4800 Bielefeld 1, FRG
Search for other works by this author on:
1 Present addresses: Department of Dairy and Food Microbiology, University College, Cork, Ireland
2 Present addresses: National Institute for Higher Education, Dublin, Ireland
Received:
21 October 1986
Accepted:
08 December 1986
Published:
12 January 1987
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Petra Grönger, Sundaram S. Manian, Helmut Reiländer, Michael O'Connell, Ursula B. Priefer, Alfred Pühler, Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fix ABC, nif A, nif B and a novel open reading frame , Nucleic Acids Research, Volume 15, Issue 1, 12 January 1987, Pages 31–49, https://doi.org/10.1093/nar/15.1.31
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Abstract
By hybridization and heteroduplex studies the fix ABC and nif A genes of the Rhizobium leguminosarum symbiotic plasmid pRL6JI have been identified. DNA sequencing of the region containing nif A showed an open reading frame of 1557 bp encoding a protein of 56,178 D. Based on sequence homology, this ORF was confirmed to correspond to the nif A gene. Comparison of three nif A proteins ( Klebsiella pneumoniaer Rhizobium meliloti, Rhizobium leguminosarum ) revealed only a weak relationship in their N-terminal regions, whereas the C-terminal parts exhibited strong homology. Sequence analysis also showed that the R. leguminosarum nif A gene is followed by nif B and preceded by fix C with an open reading frame inserted in between. This novel ORF of 294 bp was found to be highly conserved also in R. meliloti . No known promoter and termination signals could be defined on the sequenced R. leguminosarum fragment.
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Author notes
1 Present addresses: Department of Dairy and Food Microbiology, University College, Cork, Ireland
2 Present addresses: National Institute for Higher Education, Dublin, Ireland
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