Structural analysis of the mouse chromosomal gene encoding interleukin 4 which expresses B cell, T cell and mast cell stimulating activities (original) (raw)

Journal Article

,

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto

CA 94304, USA

Search for other works by this author on:

,

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto

CA 94304, USA

Search for other works by this author on:

,

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto

CA 94304, USA

Search for other works by this author on:

,

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto

CA 94304, USA

Search for other works by this author on:

,

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto

CA 94304, USA

Search for other works by this author on:

,

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto

CA 94304, USA

Search for other works by this author on:

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto

CA 94304, USA

* To whom correspondence should be addressed

Search for other works by this author on:

Revision received:

21 November 1986

Accepted:

21 November 1986

Published:

12 January 1987

• PDF
• Split View
• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

Takeshi Otsuka, Douglas Villaret, Takashi Yokota, Yutaka Takebe, Frank Lee, Naoko Arai, Ken-ichi Arai, Structural analysis of the mouse chromosomal gene encoding interleukin 4 which expresses B cell, T cell and mast cell stimulating activities, Nucleic Acids Research, Volume 15, Issue 1, 12 January 1987, Pages 333–344, https://doi.org/10.1093/nar/15.1.333
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

Based on homology with the mouse interleukin 4 (IL-4) cDNA that expresses B cell, T cell, and mast cell stimulating activities (Lee, F. et al. , (1986) Proc. Natl. Acad. Sci. USA 83 , 2061), we have isolated from a Balb/c mouse liver DNA library the mouse chromosomal gene and analyzed its overall structure. The gene occures as a single copy in the haploid genome and contains four exons and three introns. The exon sequences almost completely match the cloned cDNA sequence. Interestingly, there is a fairly high degree of homology between mouse IL-4 and mouse IL-2 genes extending more than 200 bp upstream of a “TATA” like sequence located 20 bp upstream of the transcription initiation site. These sequences may play an important role in the regulated expression of this gene in concanavalin A or antigen-stimulated T lymphocytes. The supernatant of C0S7 cells transfected with plasmid DNA containing the entire gene exhibited both T cell growth factor and mast cell growth factor activities.

This content is only available as a PDF.

© IRL Press Limited

I agree to the terms and conditions. You must accept the terms and conditions.

Submit a comment

Name

Affiliations

Comment title

Comment

You have entered an invalid code

Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.

Citations

Views

Altmetric

Metrics

Total Views 58

20 Pageviews

38 PDF Downloads

Since 2/1/2017

Citations

103 Web of Science

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic