Structural analysis of the mouse chromosomal gene encoding interleukin 4 which expresses B cell, T cell and mast cell stimulating activities (original) (raw)
Journal Article
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto
CA 94304, USA
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Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto
CA 94304, USA
Search for other works by this author on:
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto
CA 94304, USA
Search for other works by this author on:
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto
CA 94304, USA
Search for other works by this author on:
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto
CA 94304, USA
Search for other works by this author on:
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto
CA 94304, USA
Search for other works by this author on:
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto
CA 94304, USA
* To whom correspondence should be addressed
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Revision received:
21 November 1986
Accepted:
21 November 1986
Published:
12 January 1987
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Takeshi Otsuka, Douglas Villaret, Takashi Yokota, Yutaka Takebe, Frank Lee, Naoko Arai, Ken-ichi Arai, Structural analysis of the mouse chromosomal gene encoding interleukin 4 which expresses B cell, T cell and mast cell stimulating activities, Nucleic Acids Research, Volume 15, Issue 1, 12 January 1987, Pages 333–344, https://doi.org/10.1093/nar/15.1.333
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Abstract
Based on homology with the mouse interleukin 4 (IL-4) cDNA that expresses B cell, T cell, and mast cell stimulating activities (Lee, F. et al. , (1986) Proc. Natl. Acad. Sci. USA 83 , 2061), we have isolated from a Balb/c mouse liver DNA library the mouse chromosomal gene and analyzed its overall structure. The gene occures as a single copy in the haploid genome and contains four exons and three introns. The exon sequences almost completely match the cloned cDNA sequence. Interestingly, there is a fairly high degree of homology between mouse IL-4 and mouse IL-2 genes extending more than 200 bp upstream of a “TATA” like sequence located 20 bp upstream of the transcription initiation site. These sequences may play an important role in the regulated expression of this gene in concanavalin A or antigen-stimulated T lymphocytes. The supernatant of C0S7 cells transfected with plasmid DNA containing the entire gene exhibited both T cell growth factor and mast cell growth factor activities.
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