Structural features and hydration of a dodecamer duplex containing two C.A mispairs (original) (raw)
Journal Article
Search for other works by this author on:
1
Department of Chemistry, University of Edinburgh
West Mains Road, Edinburgh, UK
Search for other works by this author on:
*To whom correspondence should be addressed
Search for other works by this author on:
Revision received:
08 July 1987
Published:
25 August 1987
PDF Cite
William N. Hunter, Tom Brown, Olga Kennard, Structural features and hydration of a dodecamer duplex containing two C.A mispairs, Nucleic Acids Research, Volume 15, Issue 16, 25 August 1987, Pages 6589–6605, https://doi.org/10.1093/nar/15.16.6589
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
X-ray diffraction techniques have been used to characterise the crystal and molecular structure of the deoxyoligomer d(C-G-C-A-A-A-T-T-C-G-C-G) at 2.5Å resolution. The final R factor is 0.19 with the location of 78 solvent molecules. The oligomer crystallises in a B-DNA type conformation with two strands coiled about each other to produce a duplex. This double helix consists of four A.T and six G.C Watson-Crick base pairs and two C.A mispairs. The mismatched base pairs adopt a “wobble” type structure with the cytosine displaced laterally into the major groove, the adenine into the minor groove. We have proposed that the two close contacts observed in the C.A pairing represent two hydrogen bonds one of which results from protonation of adenine. The mispairs are accommodated in the double helix with small adjustments in the conformation of the sugar-phosphate backbone* Details of the backbone conformation, base stacking interactions, thermal parameters and the hydration are now presented and compared with those of the native oligomer d(C-G-C-G-A-A-T-T-C-G-C-G) and with variations of this sequence containing G.T and G.A mispairs.
This content is only available as a PDF.
© IRL Press Limited, Oxford, England
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.