Probing the structure of RNAs in solution (original) (raw)
Journal Article
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Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS
15 rue René Descartes, 67084 Strasbourg Cedex, France
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Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS
15 rue René Descartes, 67084 Strasbourg Cedex, France
Search for other works by this author on:
,
Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS
15 rue René Descartes, 67084 Strasbourg Cedex, France
Search for other works by this author on:
,
Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS
15 rue René Descartes, 67084 Strasbourg Cedex, France
Search for other works by this author on:
,
Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS
15 rue René Descartes, 67084 Strasbourg Cedex, France
Search for other works by this author on:
Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS
15 rue René Descartes, 67084 Strasbourg Cedex, France
*To whom correspondence should be adressed
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Revision received:
22 October 1987
Accepted:
22 October 1987
Published:
25 November 1987
PDF Cite
Chantal Ehresmann, Florence Baudin, Maryléne Mougel, Pascale Romby, Jean-Pierre Ebel, Bernard Ehresmann, Probing the structure of RNAs in solution, Nucleic Acids Research, Volume 15, Issue 22, 25 November 1987, Pages 9109–9128, https://doi.org/10.1093/nar/15.22.9109
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Abstract
During these last years, a powerful methodology has been developed to study the secondary and tertiary structure of RNA molecules either free or engaged in complex with proteins. This method allows to test the reactivity of every nucleotide towards chemical or enzymatic probes. The detection of the modified nucleotides and RNase cleavages can be conducted by two different paths which are orientated both by the length of the studied RNA and by the nature of the probes used The first one uses end-labeled RNA molecule and allows to detect only scissions in the RNA chain. The second approach is based on primer extension by reverse transcriptase and detects stops of transcription at modified or cleaved nucleotides. The synthesized cDNA fragments are then sized by electrophoresis on polyacry1amide:urea gels. In this paper, the various structure probes used so far are described, and their utilization is discussed.
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