Electroporation for the efficient transfection of mammalian cells with DNA (original) (raw)
Journal Article
,
Department of Biochemistry, Stanford University Medical Center
Stanford, CA 94305, USA
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Department of Biochemistry, Stanford University Medical Center
Stanford, CA 94305, USA
Search for other works by this author on:
Department of Biochemistry, Stanford University Medical Center
Stanford, CA 94305, USA
Search for other works by this author on:
Revision received:
02 January 1987
Accepted:
05 January 1987
Published:
11 February 1987
PDF Cite
Gilbert Chu, Hiroshi Hayakawa, Paul Berg, Electroporation for the efficient transfection of mammalian cells with DNA, Nucleic Acids Research, Volume 15, Issue 3, 11 February 1987, Pages 1311–1326, https://doi.org/10.1093/nar/15.3.1311
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Abstract
A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.
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