d(GATC) sequences influence Escherichia coli mismatch repair in a distance-dependent manner from positions both upstream and downstream of the mismatch (original) (raw)
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Friedrich Miescher Institute
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Friedrich Miescher Institute
PO Box 2543, CH-4002 Basel, Switzerland
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Friedrich Miescher Institute
PO Box 2543, CH-4002 Basel, Switzerland
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03 May 1988
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Renato Bruni, Doris Martin, Josef Jiricny, d(GATC) sequences influence Escherichia coli mismatch repair in a distance-dependent manner from positions both upstream and downstream of the mismatch , Nucleic Acids Research, Volume 16, Issue 11, 10 June 1988, Pages 4875–4890, https://doi.org/10.1093/nar/16.11.4875
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Abstract
The role of d(GATC) sites in determining the efficiency of methyl-directed mismatch repair in Escherichia coli was investigated. Transfection of host bacteria, both proficient and deficient in mismatch repair, with a series of artificially constructed M13 heteroduplexes showed that a decrease in the total number of d(GATC) sequences within these vectors lowered the efficiency of repair in vivo . Single hemimethylated d(GATC) sequences were still able to direct the correction event to the unmethylated strand, providing that the mismatch to d(GATC) site distance was shorter than approximately 1 kb. In excess of this distance, the effect of hemimethylated d(GATC) sites on mismatch correction was almost unnoticeable. The directionality of the repair event could be dictated by d(GATC) sequences situated both upstream and downstream of the mispair, suggesting that this important antimutagenic pathway can proceed bidirectionally.
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