Mismatch-containing oligonucleotide duplexes bound by the E.coli mutS -encoded protein (original) (raw)

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Friedrich Miescher Institut

PO Box 2543, CH-4002 Basel, Switzerland

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Department of Biochemistry, Duke University Medical Center

Durham, NC 27710, USA

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Friedrich Miescher Institut

PO Box 2543, CH-4002 Basel, Switzerland

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Department of Biochemistry, Duke University Medical Center

Durham, NC 27710, USA

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Revision received:

01 July 1988

Published:

25 August 1988

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Josef Jiricny, Shin-San Su, Steven G. Wood, Paul Modrich, Mismatch-containing oligonucleotide duplexes bound by the E.coli mutS -encoded protein , Nucleic Acids Research, Volume 16, Issue 16, 25 August 1988, Pages 7843–7853, https://doi.org/10.1093/nar/16.16.7843
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Abstract

The binding of the mutS gene product, a protein involved in at least two E. coli mismatch correction pathways, to a series of synthetic DNA duplexes containing mismatches or mismatch analogues of the purine/pyrimidine type was studied in order to establish whether a correlation exists between the recognition of these mispairs and the efficiency of their correction in vivo . Experiments using nitrocellulose filter binding or band-shift assays revealed that duplexes containing a G/T mismatch or its analogues I/T and DI/T were bound by the protein with affinities correlating to the efficiency of their repair in vivo . In contrast, the A/C mismatch, contained within the same sequence, was bound only poorly, despite being efficiently corrected in vivo . The analogues of the A/C mispair, uncorrected in vivo , were not detectably bound under the conditions of these assays.

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