Automated polymerase chain reaction in capillary tubes with hot air (original) (raw)
Journal Article
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Department of Pathology, University of Utah Medical School
Salt Lake City, UT 84132, USA
*To whom correspondence should be addressed.
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Department of Pathology, University of Utah Medical School
Salt Lake City, UT 84132, USA
Search for other works by this author on:
Department of Pathology, University of Utah Medical School
Salt Lake City, UT 84132, USA
Search for other works by this author on:
Received:
31 January 1989
Revision received:
10 April 1989
PDF Cite
C.T Wittwer, G C. Fillmore, D.R. Hillyard, Automated polymerase chain reaction in capillary tubes with hot air, Nucleic Acids Research, Volume 17, Issue 11, 12 June 1989, Pages 4353–4357, https://doi.org/10.1093/nar/17.11.4353
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Abstract
We describe a simple, compact, inexpensive thermal cycler that can be used for the polymerase chain reaction. Based on heat transfer with air to samples in sealed capillary tubes, the apparatus resembles a recirculating hair dryer. The temperature is regulated via thermocouple input to a programmable set-point process controller that provides proportional output to a solid state relay controlling a heating coil. For efficient cooling after the denaturation step, the controller activates a solenoid that opens a door to vent hot air and allows cool air to enter. Temperature-time profiles and amplification results approximate those obtained using water baths and microfuge tubes.
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