Automated polymerase chain reaction in capillary tubes with hot air (original) (raw)
Journal Article
,
Department of Pathology, University of Utah Medical School
Salt Lake City, UT 84132, USA
*To whom correspondence should be addressed.
Search for other works by this author on:
,
Department of Pathology, University of Utah Medical School
Salt Lake City, UT 84132, USA
Search for other works by this author on:
Department of Pathology, University of Utah Medical School
Salt Lake City, UT 84132, USA
Search for other works by this author on:
Received:
31 January 1989
Revision received:
10 April 1989
Cite
C.T Wittwer, G C. Fillmore, D.R. Hillyard, Automated polymerase chain reaction in capillary tubes with hot air, Nucleic Acids Research, Volume 17, Issue 11, 12 June 1989, Pages 4353–4357, https://doi.org/10.1093/nar/17.11.4353
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
We describe a simple, compact, inexpensive thermal cycler that can be used for the polymerase chain reaction. Based on heat transfer with air to samples in sealed capillary tubes, the apparatus resembles a recirculating hair dryer. The temperature is regulated via thermocouple input to a programmable set-point process controller that provides proportional output to a solid state relay controlling a heating coil. For efficient cooling after the denaturation step, the controller activates a solenoid that opens a door to vent hot air and allows cool air to enter. Temperature-time profiles and amplification results approximate those obtained using water baths and microfuge tubes.
This content is only available as a PDF.
© IRL Press at Oxford University
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.
Citations
Views
Altmetric
Metrics
Total Views 204
63 Pageviews
141 PDF Downloads
Since 2/1/2017
Month: | Total Views: |
---|---|
February 2017 | 4 |
March 2017 | 5 |
April 2017 | 4 |
May 2017 | 7 |
June 2017 | 4 |
August 2017 | 7 |
September 2017 | 1 |
October 2017 | 1 |
November 2017 | 5 |
December 2017 | 9 |
January 2018 | 11 |
February 2018 | 17 |
March 2018 | 25 |
April 2018 | 13 |
August 2018 | 1 |
September 2018 | 1 |
October 2018 | 1 |
November 2018 | 1 |
December 2018 | 3 |
February 2019 | 3 |
March 2019 | 1 |
September 2019 | 1 |
October 2019 | 1 |
December 2019 | 2 |
January 2020 | 3 |
April 2020 | 1 |
July 2020 | 1 |
January 2021 | 2 |
February 2021 | 1 |
March 2021 | 1 |
April 2021 | 1 |
June 2021 | 1 |
August 2021 | 1 |
September 2021 | 1 |
January 2022 | 1 |
March 2022 | 2 |
August 2022 | 3 |
September 2022 | 5 |
October 2022 | 2 |
November 2022 | 4 |
December 2022 | 4 |
April 2023 | 1 |
May 2023 | 1 |
September 2023 | 4 |
October 2023 | 4 |
November 2023 | 1 |
December 2023 | 2 |
January 2024 | 4 |
February 2024 | 2 |
March 2024 | 1 |
April 2024 | 7 |
May 2024 | 4 |
June 2024 | 2 |
July 2024 | 2 |
August 2024 | 3 |
September 2024 | 3 |
October 2024 | 1 |
Citations
131 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic