In vivo transcription of a cloned prosimian primate SINE sequence (original) (raw)
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Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center
New Orleans, LA 70112, USA
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Revision received:
30 May 1989
Accepted:
26 September 1989
Published:
11 November 1989
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Valerie K. Slagel, Prescott L. Deininger, In vivo transcription of a cloned prosimian primate SINE sequence , Nucleic Acids Research, Volume 17, Issue 21, 11 November 1989, Pages 8669–8682, https://doi.org/10.1093/nar/17.21.8669
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Abstract
SINE transcription was studied by introducing a galago Monomer family member (gal39), into the mouse Ltk − cell line. Cells transiently transfected with gal39 produce gal39-specific RNA polymerase III-directed transcripts. In permanent cell lines gal39 expression was largely shut off. Genomic environment, copy number and tandem repetition of integrated SINE sequences influenced whether or not RNA polymerase III-directed gal39 transcripts were detectable. These transcripts differ in length from the observed endogenous transcript in cultured galago cells which hybridizes to this repetitive DNA family.
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