Nuclear factors interacting with an interleukin-6 responsive element of rat α2-macroglobulin gene (original) (raw)

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,

1

Department of Preventive Medicine, Institute of Tropical Medicine, Nagasaki University

Nagasaki 852, Japan

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,

2

Research Laboratory for Genetic Information, Kyushu University

Fukuoka 812, Japan

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,

1

Department of Preventive Medicine, Institute of Tropical Medicine, Nagasaki University

Nagasaki 852, Japan

3

Department of Biochemistry, Fukuoka University School of Medicine

Fukuoka 814-01, Japan

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1

Department of Preventive Medicine, Institute of Tropical Medicine, Nagasaki University

Nagasaki 852, Japan

2

Research Laboratory for Genetic Information, Kyushu University

Fukuoka 812, Japan

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Accepted:

29 September 1989

Published:

25 November 1989

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Takashi Ito, Hiroshi Tanahashi, Yoshio Musumi, Yoshiyuki Sakaki, Nuclear factors interacting with an interleukin-6 responsive element of rat α2-macroglobulin gene, Nucleic Acids Research, Volume 17, Issue 22, 25 November 1989, Pages 9425–9435, https://doi.org/10.1093/nar/17.22.9425
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Abstract

During acute inflammation, a group of liver-derived plasma proteins, acute phase proteins (APPs), increase in concentration. Interleukin-6 (IL-6) is responsible for this increase via the induction of APP gene expression. We have identified an IL-6 responsive _cis_-acting element (IL-6RE) of gene encoding a typical APP, rat α2-macroglobulin (α2M). The IL-6RE contains a sequence that is conserved among the 5′-flanking regions of various APP genes. Introduction of mutations into the conserved sequence revealed that the sequence, termed IL-6RE core, is a critical and essential component of IL6-RE. Nuclear factors binding to the IL-6RE core were identified in livers of normal and inflamed rats. Mobility shift pattern and DNase I footprinting profile indicated that the factors from normal and inflamed stages recognized the same sequence but were distinct from each other. These results suggested that the regulation of α2M gene expression may involve mutually exclusive interaction of stage-specific _trans_-acting factors.

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