High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase (original) (raw)
Journal Article
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences
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Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences
PO Box 12233, Research Triangle Park, NC 27709, USA
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Revision received:
22 May 1990
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Kristin A. Eckert, Thomas A. Kunkel, High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase , Nucleic Acids Research, Volume 18, Issue 13, 11 July 1990, Pages 3739–3744, https://doi.org/10.1093/nar/18.13.3739
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Abstract
We demonstrate that despite lacking a 3' — 5' proofreading exonuclease, the Thermus aquaticus (Taq) DNA polymerase can catalyze highly accurate DNA synthesis in vitro . Under defined reaction conditions, the error rate per nucleotide polymerized at 70°C can be as low as 10 −5 for base substitution errors and 10 −6 for frameshift errors. The frequency of mutations produced during a single round of DNA synthesis of the lac Zα gene by Taq polymerase responds to changes in dNTP concentration, pH, and the concentration of MgCI 2 relative to the total concentration of deoxynucleotide triphosphates present in the reaction. Both base substitution and frameshift error rates of < 1/100, 000 were observed at pH 5–6 (70°C) or when MgCI 2 and deoxynucleotide triphosphates were present at equimolar concentrations. These high fidelity reaction conditions for DNA synthesis by the Taq polymerase may be useful for specialized uses of DNA amplified by the polymerase chain reaction.
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