Cell-type specific activity of two glucocorticoid responsive units of rat tyrosine aminotransferase gene is associated with multiple binding sites for C/EBP and a novel liver-specific nuclear factor (original) (raw)

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Institut Jacques Monod du CNRS, University Paris 7

Tour 43, 2 Place Jussieu, 75251 Paris Cedex 05, France

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Institut Jacques Monod du CNRS, University Paris 7

Tour 43, 2 Place Jussieu, 75251 Paris Cedex 05, France

Search for other works by this author on:

,

Institut Jacques Monod du CNRS, University Paris 7

Tour 43, 2 Place Jussieu, 75251 Paris Cedex 05, France

Search for other works by this author on:

Institut Jacques Monod du CNRS, University Paris 7

Tour 43, 2 Place Jussieu, 75251 Paris Cedex 05, France

Search for other works by this author on:

Revision received:

04 December 1990

Accepted:

04 December 1990

Published:

11 January 1991

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Thierry Grange, Jeanne Roux, Gildas Rigaud, Raymond Pictet, Cell-type specific activity of two glucocorticoid responsive units of rat tyrosine aminotransferase gene is associated with multiple binding sites for C/EBP and a novel liver-specific nuclear factor, Nucleic Acids Research, Volume 19, Issue 1, 11 January 1991, Pages 131–139, https://doi.org/10.1093/nar/19.1.131
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Abstract

The structures of two remote glucocorticoid responsive units (GRUs) that cooperatively interact to promote cell-type specific glucocorticoid induction of rat tyrosine aminotransferase gene expression have been analyzed. DNAase I footprinting and gel mobility shift analyses reveal a complex array of contiguous and overlapping sites for cell type-specific DNA binding proteins. Apart from the glucocorticoid receptor, two liver-specific nuclear factors possess multiple binding sites in each of these GRUs: C/EBP and a newly identified liver-specific factor: HNF5. C/EBP possesses four binding sites In each GRU; a DNA-binding protein with similar binding specificity has been identified in fibroblasts; this protein could be related to AP-3. HNF5 possesses two binding sites in one GRU and four in the other. There are also HNF5 binding sites in numerous regulatory regions of other liver-specific genes. The interaction of HNF5 with DNA gives a characteristic DNAase I footprint with hypersensitive sites in the middle of the recognition sequence. Some of the C/EBP and HNF5 binding sites overlap in a conserved arrangement.

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© 1991 Oxford University Press

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