Studies an chromatin. II. Isolation and characterization of chromatin subunits (original) (raw)

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a

Institute of Melecular Biology, Academy of Sciences of the USSR

Moscow B-312, USSR

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,

a

Institute of Melecular Biology, Academy of Sciences of the USSR

Moscow B-312, USSR

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,

b

Physico-Technical Institute of Low Temperatures

Kharkov, USSR

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a

Institute of Melecular Biology, Academy of Sciences of the USSR

Moscow B-312, USSR

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Published:

01 August 1975

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V.V. Bakayev, A.A. Melnickov, V.D. Osicka, A.J. Varshavsky, Studies an chromatin. II. Isolation and characterization of chromatin subunits, Nucleic Acids Research, Volume 2, Issue 8, 1 August 1975, Pages 1401–1420, https://doi.org/10.1093/nar/2.8.1401
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Abstract

Earlier findings /1–10/ bearing on a subunit organization of chromatin were confirmed and in some points detailed. Besides this, a large-scale isolation of chromatin subunits, their protein composition, electron microscopic appearance and CsCl banding pattern are described. Although the purified chromatin subunit contains all five histories, the relative content of histone H1 in it is two times lower than that in the original chromatin. It is shown that a mild digestion of chromatin with staphylococcal nuclease producesnot only separate chromatin subunits and their “oligomers” but also deoxyribonucleoprotein particles which sediment moreslowly than subunits. It appears that these particles and subunits are produced from different initial structures in the chromatin.

Finally, a crystallization of the purified chromatinsubunit as a cetyltrimethyl ammonium salt is described.

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