Elements of an archaeal promoter defined by mutational analysis (original) (raw)
Journal Article
,
Max-Planck-Institut fü Biochemie
8033 Martinsried, Germany
* To whom correspondence should be addressed
Search for other works by this author on:
,
Max-Planck-Institut fü Biochemie
8033 Martinsried, Germany
- Present address: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, USA
Search for other works by this author on:
,
Max-Planck-Institut fü Biochemie
8033 Martinsried, Germany
Search for other works by this author on:
Max-Planck-Institut fü Biochemie
8033 Martinsried, Germany
Search for other works by this author on:
- Present address: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, USA
Revision received:
25 September 1992
Accepted:
25 September 1992
Published:
25 October 1992
PDF Cite
Johannes Hain, Wolf-Dieter Reiter, Uwe Hüdepohl, Wolfram Zillig, Elements of an archaeal promoter defined by mutational analysis, Nucleic Acids Research, Volume 20, Issue 20, 25 October 1992, Pages 5423–5428, https://doi.org/10.1093/nar/20.20.5423
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
The sequence requirements for specific and efficient transcription from the 16S/23S rRNA promoter of Sulfolobus shibatae were analysed by point mutations and by cassette mutations using an in vitro transcription system. The examination of the box A-containing distal promoter element (DPE) showed the great importance of the TA sequence in the center of box A for transcription efficiency and the influence of the sequence upstream of box A on determining the distance between the DPE and the start site. In most positions of box A, replacement of the wild type bases by adenines or thymlnes are less detrimental than replacements by cytosines or guanines. The effectiveness of the proximal promoter element (PPE) was not merely determined by its high A + T content but appeared to be directly related to its nucleotide sequence. At the start site a pyrimldlne/purine (py/pu) sequence was necessary for unambiguous initiation as shown by analysis of mutants where the wild type start base was replaced. The sequence of box A optimal for promoter function in vitro is identical to the consensus of 84 mapped archaeal promoter sequences.
This content is only available as a PDF.
Author notes
- Present address: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, USA
© 1992 Oxford University Press
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.
Citations
Views
Altmetric
Metrics
Total Views 93
33 Pageviews
60 PDF Downloads
Since 1/1/2017
| Month: | Total Views: |
|---|---|
| January 2017 | 2 |
| April 2017 | 1 |
| May 2017 | 2 |
| June 2017 | 2 |
| July 2017 | 1 |
| August 2017 | 2 |
| September 2017 | 2 |
| October 2017 | 1 |
| December 2017 | 4 |
| January 2018 | 10 |
| February 2018 | 6 |
| March 2018 | 13 |
| April 2018 | 12 |
| May 2018 | 1 |
| June 2018 | 2 |
| July 2018 | 2 |
| July 2019 | 1 |
| November 2019 | 2 |
| July 2020 | 2 |
| November 2020 | 1 |
| November 2021 | 1 |
| September 2022 | 2 |
| November 2022 | 1 |
| December 2022 | 2 |
| January 2023 | 1 |
| May 2023 | 1 |
| June 2023 | 1 |
| October 2023 | 1 |
| November 2023 | 1 |
| December 2023 | 2 |
| April 2024 | 1 |
| May 2024 | 1 |
| June 2024 | 5 |
| July 2024 | 1 |
| August 2024 | 1 |
| September 2024 | 1 |
| October 2024 | 1 |
Citations
124 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic