Oligonucleotide probes detect splicing variants in situ in Drosophila embryos (original) (raw)
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Revision received:
25 September 1992
Accepted:
25 September 1992
Published:
11 November 1992
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Rubén D. Artero, Michael Akam, Manuel Pérez-Alonso, Oligonucleotide probes detect splicing variants in situ in Drosophila embryos , Nucleic Acids Research, Volume 20, Issue 21, 11 November 1992, Pages 5687–5690, https://doi.org/10.1093/nar/20.21.5687
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Abstract
We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants la and lla are similar in early embryos, but differ in late embryos. Variant IVa is only detected in the CNS (ps6) at stages 16 and 17. We also present evidence indicating that the first splicing event is cotranscriptional.
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