The MRE4 gene encodes a rovel protein kinase homologue recuired for meiotic recombination in Saccharomyces cerevisiae (original) (raw)

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Department of Biology, Faculty of Science, Osaka University

Toyonaka, Osaka 560, Japan

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Department of Biology, Faculty of Science, Osaka University

Toyonaka, Osaka 560, Japan

* To whom correspondence should be addressed

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Received:

11 November 1991

Revision received:

06 February 1992

Accepted:

06 February 1992

Published:

11 February 1992

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Sun-Hee Leem, Hideyuki Ogawa, The MRE4 gene encodes a rovel protein kinase homologue recuired for meiotic recombination in Saccharomyces cerevisiae, Nucleic Acids Research, Volume 20, Issue 3, 11 February 1992, Pages 449–457, https://doi.org/10.1093/nar/20.3.449
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Abstract

The MRE4 gene was cloned by complementation of the defects of meiotic recombination and haploidization in an mre4–1 mutant. Disruption of MRE4 resulted in reduced meiotic recombination and spore inviability. The mre4 spore Dethallity can be suppressed by spo13 , a mutation that causes cells to bypass the reductional division. Analysis of meiotic DMA extracted from the mre4 mutant cells revealed that double-strand breaks occurred at the two sites of the HIS4-LEU2 recombination hot spot, but at a frequency of about 10–20% of the wild type. Northern blot analysis indicated that the MRE4 gene produces four transcripts of 1.63, 3.2, 4.0 and 6.2 kb. ADD of these transcripts are absent from miitotic cells and are meioticalfly induced. The DNA sequence of the MRE4 open reading frame predicts a 497-amino acids protein with a molecular mass of 56.8 kDa. The Mre4 protein contains highly conserved amino acid sequences found specifically in serine-threonine protein kinases. These results suggest that protein phosphorylation is required directly or indirectly for meiotic recombination.

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