In vitro selection of fast-hybridizing and effective antisense RNAs directed against the human immunodeficiency virus type 1 (original) (raw)

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Max-Planck-Institut für Medizinische Forschung, Abteilung Biophysik, Jahnstraβe 29, D-6900 Heidelberg, Germany

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Received:

04 January 1993

Revision received:

12 February 1993

Accepted:

12 February 1993

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Karola Rittner, Christoph Burmester, Georg Sczakiel, In vitro selection of fast-hybridizing and effective antisense RNAs directed against the human immunodeficiency virus type 1, Nucleic Acids Research, Volume 21, Issue 6, 25 March 1993, Pages 1381–1387, https://doi.org/10.1093/nar/21.6.1381
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Abstract

The rate of double strand formation between procaryotlc antlsense RNA and complementary RNA In vitro Is known to correlate with the effectiveness of antlsense RNA In vivo . In this work, an In vitro assay for determining the hybridization rates of a large number of antisense RNA species was developed. A set of HIV-1-directed antlsense RNAs with the same 5′-end but successively shortened 3′-ends was produced by alkaline hydrolysis of a 150 nt HIV-1-directed antisense transcript. This mixture was used to determine hybridization rates for individual chain lengths with a complementary HI V-1-derived RNA In vitro . The second order binding rate constants of individual antlsense RNA species differed by more than a factor of 100, although In some cases, slow-hybrldlzlng and fast-hybridizing antlsense RNAs differed by only two or three 3′-termlnally-located nucleotides. The results Indicated that there was not a trivial dependence of binding rates on the chain length of antisense RNAs. Further, the binding rate constants determined In vitro for individual antisense RNA species correlated with the extent of Inhibition of HIV-1 replication In vivo .

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