Identification of the catalytic and DNA-binding region of the human immunodeficiency virus type I integrase protein (original) (raw)

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Division of Molecular Biology, The Netherlands Cancer Institute

Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands

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Antoinette A.M.Oude Groeneger

Division of Molecular Biology, The Netherlands Cancer Institute

Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands

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Division of Molecular Biology, The Netherlands Cancer Institute

Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands

* To whom correspondence should be addressed

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Received:

22 December 1992

Revision received:

02 February 1993

Accepted:

02 February 1993

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Cornelis Vink, Antoinette A.M.Oude Groeneger, Ronald H.A. Plasterk, Identification of the catalytic and DNA-binding region of the human immunodeficiency virus type I integrase protein, Nucleic Acids Research, Volume 21, Issue 6, 25 March 1993, Pages 1419–1425, https://doi.org/10.1093/nar/21.6.1419
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Abstract

The integrase (IN) protein of the human Immunodeficiency virus (HIV) is required for specific cleavage of the viral DNA termini, and subsequent integration of the viral DNA Into target DNA. To identify the various domains of the IN protein we generated a series of IN deletion mutants as fusions to maltose-binding protein (MBP). The deletion mutants were tested for their ability to bind DNA, to mediate slte-speclflc cleavage of the viral DNA ends, and to carry out Integration and disintegration reactions. We found that the DNA-blndlng region resides between amlno acids 200 and 270 of the 288-residues HIV-1 IN protein. The catalytic domain of the protein was mapped between amlno acids 50 and 194. For the specific activities of IN, cleavage of the viral DNA and Integration, both the DNA-bindlng domain and the conserved amino-termlnal region of IN are required. These regions are dispensable however, for disintegration activity.

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