Cloning and functional characterization of LCR-F1: a bZIP transcription factor that activates erythroid-specific, human globin gene expression (original) (raw)

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Department of Biochemistry and Molecular Genetics, Schools of Medicine and Dentistry, University of Alabama at Birmingham

Birmingham, AL 35294, USA

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Department of Biochemistry and Molecular Genetics, Schools of Medicine and Dentistry, University of Alabama at Birmingham

Birmingham, AL 35294, USA

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Department of Biochemistry and Molecular Genetics, Schools of Medicine and Dentistry, University of Alabama at Birmingham

Birmingham, AL 35294, USA

Search for other works by this author on:

,

Department of Biochemistry and Molecular Genetics, Schools of Medicine and Dentistry, University of Alabama at Birmingham

Birmingham, AL 35294, USA

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Department of Biochemistry and Molecular Genetics, Schools of Medicine and Dentistry, University of Alabama at Birmingham

Birmingham, AL 35294, USA

* To whom correspondence should be addressed

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Received:

02 February 1994

Revision received:

12 May 1994

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John J. Caterina, David Donze, Chiao-Wang Sun, Dominic J. Ciavatta, Tim M. Townes, Cloning and functional characterization of LCR-F1: a bZIP transcription factor that activates erythroid-specific, human globin gene expression, Nucleic Acids Research, Volume 22, Issue 12, 25 June 1994, Pages 2383–2391, https://doi.org/10.1093/nar/22.12.2383
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Abstract

DNase I hypersensitive site 2 (HS 2) of the human β-globin Locus Control Region (LCR) directs high level expression of the β-globin gene located 50 kilobases downstream. Experiments in cultured cells and in transgenic mice demonstrate that duplicated AP1-like sites in HS 2 are required for this powerful enhancer activity. A cDNA clone encoding a basic, leucine-zipper protein that binds to these sites was isolated and designated Locus Control Region-Factor 1 (LCR-F1). This protein is a member of a new family of regulatory factors that contain a 63 amino acid ‘CNC domain’ overlapping the basic region. This domain is approximately 70% identical in the Drosophila Cap N Collar (CNC) protein, NF-E2 and LCR-F1. LCR-F1 transactivates an HS 2/λ-globin reporter gene over 170-fold in transient transfection experiments specifically in erythroid cells. These results suggest that LCR-F1 may be a critical factor involved in LCR-mediated, human globin gene expression.

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