Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction (original) (raw)
Journal Article
Institut für Physikalische Biologie
Geb. 26.12.U1
Heinrich-Heine-Universität
Universitätsstraβe 1, 40225 Dusseldorf, Germany
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17 June 1994
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Gerhard Steger, Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction, Nucleic Acids Research, Volume 22, Issue 14, 25 July 1994, Pages 2760–2768, https://doi.org/10.1093/nar/22.14.2760
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Abstract
A program is described which calculates the thermal stability and the denaturation behaviour of doublestranded DNAs and RNAs up to a length of 1000 base pairs. The algorithm is based on recursive generation of conditional and a priori probabilities for base stacking. Output of the program may be compared directly to experimental results; thus the program may be used to optimize the nucleic acid fragments, the primers and the experimental conditions prior to experiments like polymerase chain reactions, temperature-gradient gel electrophoresis, denaturinggradient gel electrophoresis and hybridizations. The program is available in three versions; the first version runs interactively on VAXstations producing graphics output directly, the second is implemented as part of the HUSAR package at GENIUSnet, the third runs on any computer producing text output which serves as input to available graphics programs.
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