The expression pattern of Id4, a novel dominant negative helix-loop-helix protein, is distinct from Id1, 1d2 and Id3 (original) (raw)
Journal Article
,
Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft Carl-von-Linné-Weg
10, 50829 Köln, Germany
Search for other works by this author on:
,
Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft Carl-von-Linné-Weg
10, 50829 Köln, Germany
Search for other works by this author on:
Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft Carl-von-Linné-Weg
10, 50829 Köln, Germany
*To whom correspondence should be addressed
Search for other works by this author on:
Received:
20 December 1993
Cite
Veit Riechmann, Ingeborg van Crüchten, Fred Sablitzky, The expression pattern of Id4, a novel dominant negative helix-loop-helix protein, is distinct from Id1, 1d2 and Id3, Nucleic Acids Research, Volume 22, Issue 5, 11 March 1994, Pages 749–755, https://doi.org/10.1093/nar/22.5.749
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
Molecular Interaction between transcription factors containing an baslc-hellx-loop-hellx (bHLH) domain is known to regulate differentiation In several cellular systems Including myogenesls, neurogenesis and haematopoiesls. DNA-blnding activity of the bHLH proteins Is mediated via the basic region and is dependent upon formation of homo- and/or heterodlmers of these transcription factors. Dominant negative (dn) HLH proteins (Id1, Id2, Id3 and emc) also contain the HLH-dimerization domain but lack the DNA binding basic region. Formation of heterodlmers between dnHLH and bHLH proteins abolishes the DNA blndlng activity of the latter. Concordantly, it was shown that the dnHLH protein Id1 Inhibits differentiation of muscle and myeloid cells in vitro. Therefore, it was postulated that dnHLH proteins serve as general antagonists of cell differentiation. We have isolated and characterized a novel mouse dnHLH gene, designated Id4. The Id4 protein contains a HLH domain highly conserved among the dnHLH proteins from mouse and drosophlla. Outside of the HLH domain, three additional short regions of the dnHLH proteins show some degree of homology. DNA-bindlng of E47 homo- as well as E47/MyoD heterodimers is Inhibited by Id4. Transcription of the Id4 gene results In three RNA molecules of 3.7, 2.0 and 1.7 kb which are presumably a result of differential splicing and/or alternatively used poly adenylation sites within the 3′ untranslated region. During embryogenesis, Id4 expression is up-regulated between day 9.5 and 13.5 of gestation. The highest expression In adult tissues was detected in testis, brain and kidney. Comparison of the expression patterns of the four mouse dnHLH genes revealed that Id4 expression differs from the more restricted expression of Id2 as well as from the widespread expression of Id1 and Id3.
This content is only available as a PDF.
© Oxford University Press
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.
Citations
Views
Altmetric
Metrics
Total Views 243
52 Pageviews
191 PDF Downloads
Since 2/1/2017
Month: | Total Views: |
---|---|
February 2017 | 6 |
March 2017 | 3 |
April 2017 | 6 |
May 2017 | 3 |
June 2017 | 3 |
July 2017 | 2 |
August 2017 | 5 |
September 2017 | 1 |
October 2017 | 1 |
November 2017 | 5 |
December 2017 | 11 |
January 2018 | 8 |
February 2018 | 13 |
March 2018 | 8 |
April 2018 | 14 |
May 2018 | 1 |
June 2018 | 2 |
July 2018 | 2 |
September 2018 | 1 |
November 2018 | 1 |
January 2019 | 1 |
May 2019 | 1 |
June 2019 | 2 |
July 2019 | 1 |
September 2019 | 2 |
October 2019 | 1 |
December 2019 | 1 |
January 2020 | 2 |
February 2020 | 1 |
July 2020 | 1 |
September 2020 | 1 |
February 2021 | 1 |
March 2021 | 2 |
April 2021 | 1 |
June 2021 | 1 |
July 2021 | 1 |
August 2021 | 4 |
October 2021 | 1 |
November 2021 | 2 |
December 2021 | 1 |
January 2022 | 1 |
May 2022 | 3 |
June 2022 | 2 |
July 2022 | 3 |
August 2022 | 3 |
September 2022 | 2 |
October 2022 | 3 |
January 2023 | 3 |
March 2023 | 1 |
April 2023 | 2 |
June 2023 | 2 |
July 2023 | 1 |
August 2023 | 1 |
September 2023 | 3 |
October 2023 | 5 |
November 2023 | 3 |
December 2023 | 4 |
January 2024 | 6 |
February 2024 | 10 |
March 2024 | 5 |
April 2024 | 8 |
May 2024 | 8 |
June 2024 | 12 |
July 2024 | 22 |
August 2024 | 3 |
September 2024 | 1 |
October 2024 | 2 |
Citations
247 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic