Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase (original) (raw)

Journal Article

,

1

Laboaory of Molecular Genetics University of Tokyo

4-6-1 Shirokanedi, Minato-ku, Tokyo 108, Japan

Search for other works by this author on:

,

1

Laboaory of Molecular Genetics University of Tokyo

4-6-1 Shirokanedi, Minato-ku, Tokyo 108, Japan

Search for other works by this author on:

,

1

Laboaory of Molecular Genetics University of Tokyo

4-6-1 Shirokanedi, Minato-ku, Tokyo 108, Japan

Search for other works by this author on:

,

1

Laboaory of Molecular Genetics University of Tokyo

4-6-1 Shirokanedi, Minato-ku, Tokyo 108, Japan

Search for other works by this author on:

,

1

Laboaory of Molecular Genetics University of Tokyo

4-6-1 Shirokanedi, Minato-ku, Tokyo 108, Japan

3

Discovery Research Laboratories III, Sumitomo Pharmaceuticals Research Center

3-1-98 Kasugade-naka, Konohana-ku, Osaka 554, Japan

Search for other works by this author on:

,

3

Discovery Research Laboratories III, Sumitomo Pharmaceuticals Research Center

3-1-98 Kasugade-naka, Konohana-ku, Osaka 554, Japan

Search for other works by this author on:

,

2

Deparment of Virology, institute of Medical Science, University of Tokyo

4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan

Search for other works by this author on:

1

Laboaory of Molecular Genetics University of Tokyo

4-6-1 Shirokanedi, Minato-ku, Tokyo 108, Japan

* To whom correspondence should be addressed

Search for other works by this author on:

Published:

11 October 1995

• PDF
• Split View
• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

Yumi Kanegae, Gwang Lee, Yumi Sato, Mieko Tanaka, Michio Nakal, Toshiyuki Sakaki, Sumio Sugano, Izumu Saito, Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase, Nucleic Acids Research, Volume 23, Issue 19, 11 October 1995, Pages 3816–3821, https://doi.org/10.1093/nar/23.19.3816
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian colls, another recombinant Ad bearing an on/off-switching reporter unit, where a LacZ -expression unit can be activated by the Cre-mediated exclsionai deletion of an interposed stuffer DNA, was also constructed. Co-infection experiments together with the Cre-expressing and the reporter recombinant Ads showed that the Cre-mediated switching of gene expression was detected in nearly 100% of cultured CV1, HeLa and Jurkat cells. These results suggest that the recombinant Ad efficiently expressed functional Cre and offers a basis for estabilshing a powerful on/off switching strategy of gene expression incultured mammalian cells and presumably in transgenic animals. The method is also applicable to construction of recombinant Ad bearing a gene the expression of which is deleterious to propagation of recombinant Ad.

This content is only available as a PDF.

© 1995 Oxford University Press

I agree to the terms and conditions. You must accept the terms and conditions.

Submit a comment

Name

Affiliations

Comment title

Comment

You have entered an invalid code

Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.

Citations

Views

Altmetric

Metrics

Total Views 322

145 Pageviews

177 PDF Downloads

Since 11/1/2016

Citations

608 Web of Science

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic